烟曲霉孢子对人肺泡上皮细胞早期凋亡的影响  

Aspergillus fumigatus conidia inhibit early apoptosis of alveolar epithelial cells

作　　者：孙禾[1] 吴婷[2] 徐小勇[2] 苏欣[2] 施毅[2] 

机构地区：[1]上海交通大学附属第一人民医院呼吸科,200080 [2]南京大学医学院临床学院南京军区南京总医院呼吸与危重症医学科

出　　处：《中华传染病杂志》2015年第7期420-423,共4页Chinese Journal of Infectious Diseases

摘　　要：目的观察烟曲霉孢子对人肺泡上皮细胞早期凋亡的影响。方法TNF—α或星孢霉素（STS）诱导人肺腺癌A549细胞凋亡。用三种浓度（5×10^5、5×10^6、5×10^7）的烟曲霉孢子与上述细胞共培养，流式细胞仪检测细胞凋亡率的变化。结果TNF-α与未刺激空白对照组比较，刺激后A549细胞的早期凋亡率增高[（14．94±0．82）％比（3．26±0．74）％，x^2=9．18，P〈0．05]STS刺激后A549细胞的早期凋亡率也明显增加[（18．10±1．00）％比（3．26±0．74）％，x^2=12．32，P〈0．053，加入不同浓度的烟曲霉孢子后，A549细胞的早期凋亡率均明显下降（x^2=31．23，P〈0．05），细胞的早期凋亡率与烟曲霉孢子浓度呈负相关（rTNF=-0．92，rSTS=-0．94，均P〈0．05）。结论烟曲霉孢子抑制由TNF-α或STS诱导的人肺腺癌A549细胞的早期凋亡；且烟曲霉孢子浓度越高，抑制作用越明显。Objective To observe the impact of Aspergillus fumigatus conidia on the early apoptosis of alveolar epithelial cells. Methods A549 cells was first stimulated with tumor necrosis factor- α （TNF-α） or staurosporine （STS） to induce cell apoptosis, and then co-cultured with Aspergillus fumigatus conidia of three different concentrations （5× 10^5, 5 × 10^6 , 5 × 10^7 ） simultaneously. The changes of the apoptosis rate of A549 cells were detected by flow cytometry. Results The early apoptosis of A549 cells was increased markedly after stimulated with TNF-α （[14. 94 ± 0. 82]% vs [-3. 26 ± 0.74]%, x^2 9.18, P〈0.05） and STS （[18.10±1.00]% vs [3.26±0.74]%, ）x^2 =12.32, P〈0.05）. When Aspergillus fumigatus conidia of different concentrations were added, the apoptosis rate was decreased （x^2 =31.23, P〈0.05）. The early apoptosis of A549 cells was negatively correlated with the concentration of Aspergillus fumigatus conidia （ rTNF = - 0. 92, rTNF = - 0. 94, both P 〈 0. 05 ）. Conclusion Aspergillus fumigatus conidia inhibits the early apoptosis of A549 ceils induced by TNF-α or STS and the inhibit effect increases with the concentration of Aspergillus fumigatus conidia.

关 键 词：曲霉菌 烟 孢子 真菌 肿瘤坏死因子Α 星状孢子素 上皮细胞 细胞凋亡 

分 类 号：R519[医药卫生—内科学]