维生素C对普安银鲫早期发育中氧化损伤及总抗氧化能力的影响  

Effect of Vitamin C on Oxidative Damage and Total Antioxidant Capacity in Early Development of Carassius auratusgibelio

作　　者：姚俊杰[1] 熊铧龙[1] 蒋左玉 安苗[1] 朱忠胜[1] 

机构地区：[1]贵州大学动物科学学院水产科学系,贵阳550025

出　　处：《动物学杂志》2015年第4期581-590,共10页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.31160527)

摘　　要：本实验旨在探究普安银鲫(Carassius auratus gibelio)早期发育过程中氧化损伤程度和总抗氧化能力的变化,及添加维生素C(Vc)后对它们的影响。采用生化方法测定了普安银鲫早期发育过程中丙二醛(MDA)含量、蛋白质羰基(PCA)含量、乳酸脱氢酶(LDH)的活性和总抗氧化能力(T-AOC)。(1)30 mg/L的Vc溶液中仔鱼孵化率和成活率最高,分别高出对照组(Vc浓度为0 mg/L)16.2%和17.8%;(2)普安银鲫早期发育过程中丙二醛、蛋白质羰基含量及乳酸脱氢酶活性均呈"升高-降低"的趋势,均在原肠中期达到最高,总抗氧化能力逐渐增加;(3)30 mg/L的Vc组中丙二醛、蛋白质羰基含量及乳酸脱氢酶的活性显著低于对照组(P<0.05),总抗氧化能力显著高于对照组(P<0.05)。结果表明,(1)普安银鲫胚胎期受到的氧化损伤比胚后严重;(2)适宜浓度的维生素C能提高胚胎及仔鱼的抗氧化能力,有效降低胚胎及仔鱼受到的氧化损伤,提高其孵化率及存活率。The experiment was conducted to study the contents of malondialdehyde（MDA） and protein carbonyl（PCA）, the activity of lactic dehydrogenase（LDH） and the total antioxidant capacity（T-AOC） as well as the effects of glucose and vitamin C on them indexes during early development of Carassius auratus gibelio. In order to improve the hatching rate and survival rate of larva, the research takes C. a. gibelio as studying objects, and different concentrations of glucose solution and vitamin C solution were added to hatching water, respectively, until the yolk sac disappeared completely. The concentrations of glucose were 5 g/L, 10 g/L, 15 g/L, and 20 g/L, and the concentrations of vitamin C were 20 mg/L, 25 mg/L, 30 mg/L and 35 mg/L, respectively. The incubation time, hatching rate and survival rate of larvae were recorded during the process of experiment, and the contents of malondialdehyde and protein carbonyl, the activity of lactic dehydrogenase and the total antioxidant capacity during early development of C. a. gibelio were analyzed by enzymological method and data were analyzed by T-test and one way ANOVA. The results showed that：（1） The hatching rate and survival rate of larva in vitamin C group were higher than those of control group, the hatching rate and survival rate were highest when the concentration of vitamin C was 30 mg/ L, and they 16.2% and 17.8% higher than those of control group, respectively（Table 1）.（2） The contents of malondialdehyde and protein carbonyl, the activity of lactic dehydrogenase showed a ＂up-down＂ trend, and these three indexes reached the highest in mig-gastrocoel stage, while the total antioxidant capacity increased during early development of C. a. gibelio.（3） In the group of vitamin C（30 mg/L）, the contents of malondialdehyde and protein carbonyl and the activity of lactic dehydrogenase were significantly lower than those of control group, while the total antioxidant capacity was significantly higher than that of control group（P〈0.05）�

关 键 词：维生素C 普安银鲫 早期发育 氧化损伤 总抗氧化能力 

分 类 号：S965.117[农业科学—水产养殖]