一测多评法测定泽泻中4种三萜类成分的含量  

Simultaneous Quantitative Analysis of Four Triterpenoids in Alismatis Rhizoma by Quantitative Analysis of Multi-components by Single Marker

作　　者：赵万里[1] 张方方[1] 黄小强[1] 罗奋熔 谌赛男 李小艳[1] 许文[1,2] 吴水生[1] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建中医药大学大学生物医药研发中心,福州350122

出　　处：《中国药学杂志》2015年第15期1347-1352,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(U1205022);国家"十二五"科技支撑计划(2011BAI01B06);福建省自然基金资助项目(2014J01352);福建省卫生厅青年科研资助课题(2013-2-55);福建中医药大学校管课题(X2014107-学科X2015011-平台);福建省大学生创新训练项目(201410393034)

摘　　要：目的建立一测多评(QAMS)的泽泻多指标质量控制方法 ,并验证该方法在泽泻含量测定中应用的可行性和技术适应性。方法采用HPLC,以23-乙酰泽泻醇B为内参物,计算泽泻醇A、24-乙酰泽泻醇A和泽泻醇B的相对校正因子,并利用该校正因子计算含量,同时与采用外标法测定4个三萜类成分的含量进行比较。结果建立的校正因子重现性良好,31批泽泻药材中4个三萜类的含量计算值与外标法实测值间(RSD≤3.52%)无显著差异。结论本实验所建立的一测多评法稳定、准确,可用于泽泻三萜类成分的质量控制。OBJECTIVE To develop a simultaneous quantitative analysis method of four triterpenoids in Alismatis Rhizoma by multi-components by single maker （QAMS）. METHODS Four main triterpenoids （ alisol A, alisol A 24-acetate, alisol B and alisol B 23-acetate） were selected as analytes to evaluate the quality of Alismatis Rhizoma. Alisol B 23-acetate was used as the internal refer- ence standard and three relative correction factors （RCFs） to alisol B 23-acetate were calculated. These RCFs were obtained by high performance liquid chromatography coupled with diode array detector under various chromatographic conditions. Then 31 batches of A1- ismatis Rhizoma samples collected from different areas were determined by both external standard method （ESM） and QAMS. RE- SULTS The RCFs were obtained with good reproducibility （ RSD ≤ 2.25% ）. No obvious differences were observed between the analysis results of QAMS method and ESM method （ RSD ≤3.52% ）. CONCLUSION The established QAMS method is reliable and feasible for simultaneous analysis of four triterpenoids and could be used for quality control of Alismatis Rhizoma. KEY WORDS ： Alismatis Rhizoma ; triterpenoids ; QAMS ; quality control

关 键 词：泽泻 三萜 一测多评 质量控制 

分 类 号：R917[医药卫生—药物分析学]