拟南芥钙依赖型蛋白激酶突变体cpk3-1和cpk6-1 PCR鉴定及其在CO2通路中的作用  

Identification of Arabidopsis Thaliana Calcium-Dependent Protein Kinase cpk3-1,cpk6-1 and cpk3-1/6-1 Mutant Using PCR and the Roles in CO_2 Signalling Pathway

作　　者：王蕾[1] 张显文[2] 田保华[3] 薛绍武[2] 

机构地区：[1]山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,山西太原030006 [2]华中农业大学生命科学技术学院,湖北武汉430070 [3]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2015年第3期527-533,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：中央高校基本科研业务费专项资金(2014PY065);湖北省自然科学基金(2014CFB949);华中农业大学科研启动费(2013RC012)

摘　　要：植物中钙依赖性蛋白激酶(Calcium-dependent protein kinases,CDPK)是一种重要的感受Ca2+流的传感器,广泛参与到气孔运动和响应各种胁迫刺激的信号转导途径中。研究通过获得拟南芥纯合突变体cpk3-1,cpk6-1和cpk3-1/cpk6-1,研究了CPK3与CPK6在CO2信号通路中作用。结果显示,编码离子通道的基因在不同浓度的CO2处理时表达量会发生变化,但在突变体cpk3-1,cpk6-1,cpk3-1/cpk6-1中,与野生型相比,这些基因的表达量没有差异,表明在CO2信号通路里,CPK3和CPK6可能不发挥作用。Calcium-dependent protein kinases（CDPKs）,which are widely involved in stomatal movement and response to various environmental stresses,are essential sensors of Ca^2＋flux in plant.Homozygous mutants cpk3-1,cpk6-1,cpk3-1/cpk6-1in Arabidopsis thaliana were indentified and it was investigated whether CPK3 and CPK6have function in CO2 signal pathway.The results showed that the expressions of the genes encoding ion channel were significantly different when wild-type and mutants plants were treated with different concentrations of CO2,but the expressions of the genes have no significantly different compared the mutants cpk3-1,cpk6-1,cpk3-1/cpk6-1 with wild-type plant.CPK3 and CPK6 may have no function in the CO2 signaling pathway.

关 键 词：拟南芥 T-DNA插入突变 钙依赖型蛋白激酶 CO2 

分 类 号：Q945.78[生物学—植物学]