罗格列酮对 UVB 诱导的小鼠光老化皮肤成纤维细胞MMPs表达和细胞外基质蛋白合成的影响  

Effects of rosiglitazone on the MMPs and ECM in UVB induced photo-aging mouse fibroblasts

作　　者：陈亮[1] 毕波[1] 曾继平[1] 刘天一[1] 朱宁文[2] 杨清建 贾传龙 卢勇舟 周轶群 杨平[1] 

机构地区：[1]复旦大学附属华东医院整形外科,上海200040 [2]复旦大学附属华山医院皮肤科

出　　处：《中国美容整形外科杂志》2015年第8期500-503,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金资助项目（81272125;8130164-2）;上海市卫生系统优秀学科带头人培养计划资助项目（XBR2011033）;863基金资助项目（SS2014AA020705）

摘　　要：目的：探讨罗格列酮（ RO）对光老化皮肤成纤维细胞（ FBs）基质金属蛋白酶（ MMPs）表达和细胞外基质（ ECM）蛋白合成的影响。方法采用cck-8法检测RO对FBs活性的影响。 UVB反复照射诱导产生体外细胞光老化模型。造模结束24 h,Real-Time PCR检测MMPs的表达。造模结束48 h, western blotting检测弹性蛋白原和Ⅰ型胶原的表达。结果低剂量RO对FBs的细胞活性无影响。 UVB诱导的光老化FBs中,MMP-1,2,3,7和9的表达均显著升高。40 uM RO能降低UVB引起的MMPs过表达（ P 〈0.05）；同时,能在蛋白水平上减缓 UVB 对 FBs 弹性蛋白原和Ⅰ型胶原合成的抑制（ P 〈0.05）。结论 RO可降低光老化FBs中MMPs的表达,增加弹性蛋白原和Ⅰ型胶原的合成,维持光老化皮肤ECM成分的稳定,可作为对抗光老化的新手段。Objective To investigate the influence of Rosiglitazone （ RO ） on the expression of matrix metalloproteinases （ MMPs ） and the synthesis of dermal extracellular matrix （ ECM ） proteins in photo-aging fibroblasts （FBs） induced by UVB. Methods The influence of RO on FBs proliferation was measured by cck 8 as-say. The photo-aging model of FBs was induced by repeated UVB radiation. The influence of RO on the expression of MMPs of photo-aging mice FBs was evaluated by Real-Time PCR at 24 h after the last radiation and the synthesis of collagen I and tropoelastin were detected by western blotting at 48 h after the last radiation. Results No modifica-tion in the proliferative activity of FBs was observed at any dose tested up to 80μM after 48 h. The UVB increased the expressions of MMP-1, 2, 3, 7 and 9 of FBs. But RO pretreatment inhibited the increasing expression of MMPs compared with the UVB group and the synthesis of tropoelastin and collagen I were increased by RO pretreatment in UVB＋RO group compared with UVB group. Conclusion RO can maintain the metabolic balance of dermal ECM by decreasing the expression of MMPs and increasing the synthesis of tropoelastin and collagen I in photo-aging FBs. The novel application of RO may lead to innovative and effective anti-photoaging therapies.

关 键 词：罗格列酮 光老化 成纤维细胞 MMPS ECM 

分 类 号：R622[医药卫生—整形外科]