Cloning and identification of an Ubiquitinconjugating enzyme E2 D2 gene from Japanese lamprey Lampetra japonica  

作　　者：Liyong Chen Fenfang Wu Bo Feng 

机构地区：[1]Guangdong Province Key Laboratory for Medical Molecular Diagnostics, China-America Cancer Research Institute, Guangdong Medical College [2]State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Department of Biochemistry, College of Life Sciences, Sun Yat-Sen (Zhongshan) University

出　　处：《生物技术世界》2015年第3期63-65,共3页Biotech World

摘　　要：The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.Ubiquitin-conjugating enzyme E2 D2 is a protein that is encoded by the UBE2D2 gene.Here,we report a lamprey(La UBE2D2)gene which contained 441-bp open reading frame(ORF)encoding 147 amino acids with a typical UBC domain.Real-time PCR assay showed that the highest expression of the protein in adult lamprey was in the leukocytes,the lowest expression was in the skin,kidney and liver.The high conservation in amino acid sequence of the La UBE2D2protein with the UBE2D2s from Homo sapiens,Danio rerio,Oreochromis niloticus and Takifugu rubripes,implied that it had similar function with UBE2D2proteins from other species.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.Ubiquitin-conjugating enzyme E2 D2 is a protein that is encoded by the UBE2D2 gene. Here, we report a lamprey(La UBE2D2) gene which contained 441-bp open reading frame(ORF) encoding 147 amino acids with a typical UBC domain. Real-time PCR assay showed that the highest expression of the protein in adult lamprey was in the leukocytes, the lowest expression was in the skin, kidney and liver. The high conservation in amino acid sequence of the La UBE2D2 protein with the UBE2D2 s from Homo sapiens, Danio rerio, Oreochromis niloticus and Takifugu rubripes, implied that it had similar function with UBE2D2 proteins from other species.

关 键 词：Ubiquitin-conjugating enzymes  UBE2D2  ubiquitin  Lamprey. 

分 类 号：S9[农业科学—水产科学]