微波法提取石蒜中石蒜碱的工艺优化  

Process Optimization of Microwave Extraction of Lycorine from Lycoris herb

作　　者：文卓琼[1,2] 蒋利华[3] 熊海蓉[1] 熊远福[4] 胡红远 刘祺文 

机构地区：[1]湖南农业大学分析测试中心,长沙410128 [2]武昌工学院,武汉430065 [3]长沙环境保护职业技术学院,长沙410004 [4]湖南农业大学理学院,长沙410128

出　　处：《中国农学通报》2015年第24期247-251,共5页Chinese Agricultural Science Bulletin

基　　金：湖南省科技厅社会发展支撑计划项目"石蒜主要药用成分提取与纯化关键技术研究"(2011SK3082)

摘　　要：为了优化微波法提取石蒜中石蒜碱的工艺条件,用野生石蒜作原料,以乙醇为提取剂,探索了料液比、微波功率、提取温度、提取时间、提取次数等单因素对微波法提取石蒜碱的影响,用正交试验L9(34)获得了最佳提取工艺条件,并与常规溶剂法进行了比较。结果表明:微波法提取石蒜中石蒜碱的最佳工艺条件为:料液比1:6,微波功率320 W,提取温度60℃,提取时间6 min,提取2次;石蒜碱的提取率为95.5%,产率为0.0391%;提取物中石蒜碱含量为12.35%。与常规溶剂法相比,微波法具备提取时间短、提取率高、提取次数少等优点,总体效果优于常规溶剂法。To optimize the process conditions of microwave extraction of lycorine from wild Lycoris herb, the single factor experiments of the solid-liquid ratio, microwave watt, extraction temperature, extraction time and extraction times were made by means of using wild Lycoris herb as raw material and ethanol as extractant, and the optimal technics conditions of the extraction were obtained by orthogonal experiment L9（3^4）. Effects of the microwave extraction were compared with those of conventional solvent extraction. Results showed that the optimal conditions of microwave extraction were as follows： the ratio of sample to solvent was 1：6 （g：mL）, microwave watt 320 W, extraction temperature 60℃, extraction time 6 rain, and extraction 2 times. The extraction ratio and yield of lycorine were 95.5% and 0.0391%, respectively. The concentration of lycorine in the extract was 12.35%. Compared with the conventional solvent method, the microwave method had advantages of less extraction time, higher extraction ratio and less extraction times. The general effect of microwave method was better than that of conventional solvent method.

关 键 词：石蒜 石蒜碱 提取工艺 微波法 常规溶剂法 

分 类 号：R284.2[医药卫生—中药学]