高分子大孔微球的制备和结构控制  

Preparation and structure control of macroporous polymer microspheres

作　　者：周炜清[1] 李娟[1] 那向明[1] 马光辉[1] 

机构地区：[1]中国科学院过程工程研究所国家生化工程重点实验室,北京100190

出　　处：《化工学报》2015年第8期2846-2853,共8页CIESC Journal

基　　金：国家自然科学基金项目(21336010);国家高技术研究发展计划项目(2012AA02A406;2012AA021505)~~

摘　　要：高分子大孔微球的形成和结构调控是一个复杂的过程,特别对于孔径超过100 nm的超大孔微球而言,无法通过常规的制孔剂与聚合物之间的相分离得到。针对生化工程对于超大孔微球的重要需求,发展了反胶团溶胀法和复乳法,实现了孔径在百纳米级以上的控制。研究了微球结构对于应用效果的影响。超大孔微球用于生物大分子的分离纯化,显示出高载量、高活性回收率、高纯化倍数的特点。超大孔微球固定化酶在酶的热稳定性、储存稳定性、重复使用性能等方面具有显著的优势。微球的结构有效控制是在不同领域获得成功应用的保障。Formation and structure control of macroporous microsphere is a complex process. Special techniques were developed for the preparation of gigaporous microspheres with large pore size over 100 nm. To meet the needs of gigaporous microsphere in biochemical engineering, the reverse micelles swelling method and the multiple emulsion method were developed which achieved the control of pore diameter above 100 nm. The effects of microsphere structures on their applications were also studied. When used in separation and purification of biomacromolecule, the gigaporous microspheres showed much higher capacity, activity recovery and purification fold. Immobilized enzymes in gigaporous microsphere also have some significant advantages in thermal stability, storage stability and reusability. The effective control of the microsphere structure is important for success application in different fields.

关 键 词：大孔微球 结构控制 药物缓控释 分离纯化 

分 类 号：TQ316.3[化学工程—高聚物工业]