HPLC法测定依托度酸胶囊的含量  

Determination of etodolac in Etodolac Capsules by HPLC

作　　者：王增日 

机构地区：[1]鲁南制药集团股份有限公司,山东临沂276000

出　　处：《药学研究》2015年第8期451-452,471,共3页Journal of Pharmaceutical Research

摘　　要：目的建立高效液相色谱法测定依托度酸胶囊中依托度酸含量的方法。方法采用高效液相色谱法,色谱柱为Diamonsil C18柱（4.6 mm×150 mm,5μm）;乙腈：水＋磷酸[520∶480（500＋0.25）]为流动相,流速1.5m L·min-1,检测波长为274 nm。结果在选定的色谱条件下依托度酸胶囊中辅料对主药无干扰,依托度酸在24.98~199.8μg·m L-1浓度范围内峰面积与浓度线性关系良好,相关系数r=0.999 9,平均回收率为99.6%,RSD为0.47%（n=9）。结论本方法简便、准确,专属性强,可用于依托度酸胶囊中依托度酸的含量测定。Objective To establish an HPLC method for the determination of etodolac in Etodolac Capsules. Methods HPLC method was adopted with Diamonsil C18column（ 4. 6 mm × 150 mm,5 μm）. A mixture of acetonitrile： water ＋phosphoric acid[520∶480（ 500 ＋ 0. 25） ] was used as the mobile phase. The flow rate was 1. 5 m L·min- 1with the detection wavelength at 274 nm. Results At the validated method,the excipient had no interference to the principal agent. A linear range of etodolac was 24. 98 ~ 199. 8 μg·m L- 1（ r = 0. 999 9）. The average recovery was 99. 6% with RSD = 0. 47%（ n = 9）. Conclusion This method was simple,accurate,specific and could be used for the determination of etodolac in Etodolac Capsules.

关 键 词：依托度酸 高效液相色谱法 含量测定 胶囊 

分 类 号：R927.2[医药卫生—药学]