亚麻木酚素抑制C_(57)BL/6小鼠Lewis肺癌生长及肺转移  

Secoisolariciresinol diglucoside suppresses tumor growth and lung metastasis of Lewis lung carcinoma in C_(57)BL /6 mice

作　　者：郑书国[1] 杨慧[1] 王宏婷[1] 杨解人[1] 

机构地区：[1]皖南医学院药理学教研室

出　　处：《中国临床药理学与治疗学》2015年第7期745-749,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察亚麻木酚素(SDG)对C57BL/6小鼠Lewis肺癌生长及肺转移的影响,并探讨其可能机制。方法:将Lewis肺癌细胞接种于C57BL/6小鼠腋下,制备小鼠肺癌模型。灌胃给予SDG(0.11,0.33,1.00 g/kg)21 d后,剥离腋下瘤块并称重;摘取肺脏,计数肺表面转移瘤结节数;HE染色法观察肺组织病理学变化,免疫组化法检测肺组织基质金属蛋白酶-2(MMP-2)、MMP-9蛋白表达水平,RT-PCR法检测肺组织细胞外基质金属蛋白酶诱导因子(EMMPRIN)mRNA表达水平。结果:SDG能明显抑制C57BL/6小鼠腋下肿瘤生长及肺转移(P<0.01),减轻肺组织病理改变,降低肺组织EMMPRIN mRNA和MMP-2、MMP-9蛋白表达水平(P<0.05或P<0.01)。结论:SDG显著抑制C57BL/6小鼠Lewis肺癌生长及肺转移,其机制与抑制EMMPRIN mRNA表达,进而抑制MMP-2、MMP-9蛋白表达有关。AIM： To observe the effect of secoisolariciresinol diglucoside（ SDG） on tumor growth and lung metastasis of Lewis lung carcinoma in C57 BL /6 mice and the possible mechanisms involved.METHODS： Lewis lung carcinoma model was established in C57 BL /6 mice by subaxillary injection of Lewis lung carcinoma cells. After supplementation with SDG for 21 d,subaxillary tumors were dissected and weighted. Lungs were excised and examined for metastatic tumor nodes and pathological changes.Protein expression of matrix metalloproteinase-2（ MMP-2） and MMP-9 were assayed by immunohistochemistry. Levels of extracellular matrix metalloproteinase inducer（ EMMPRIN） mRNA in lungs were determined by RT-PCR. RESULTS： SDG significantly suppressed subaxillary tumor growth and lung metastasis in C57 BL /6 mice（ P〈0. 01）,with the pathological changes in lungs alleviated evidently. Expressions of MMP-2 and MMP-9 protein and EMMPRIN mRNA were decreased markedly in SDG supplemented groups（ P〈0. 05 or P〈0. 01）.CONCLUSION： SDG significantly suppressed tumor growth and lung metastasis of Lewis lung carcinoma in C57 BL /6 mice,which might be attributed to inhibition of EMMPRIN mRNA expression and subsequent MMP-2 and MMP-9 protein expression.

关 键 词：亚麻木酚素 LEWIS肺癌 基质金属蛋白酶 植物雌激素 

分 类 号：R965.2[医药卫生—药理学]