单采血小板的储存时间对其代谢功能影响的研究  

Effect of storage time on metabolic function of apheresis platelets

作　　者：宋自阆[1] 兰培相[1] 张洪为[2] 

机构地区：[1]四川自贡市第四人民医院输血科,643000 [2]四川泸州医学院附属医院输血科,646000

出　　处：《国际输血及血液学杂志》2015年第4期300-302,共3页International Journal of Blood Transfusion and Hematology

基　　金：基金项目：中国高校医学期刊临床专项资金（11521769）

摘　　要：目的探讨单采血小板的储存时间对其代谢功能的影响。方法选择四川自贡市中心血站向医院提供的新鲜单采血小板悬液30袋，每袋包含1治疗量血小板，每袋血小板含量为（2．5～3．0）×10^11／袋为研究对象。将其编号后按照数字表法随机平均分为：保存1d组、保存3d组及保存5d组，每组各为10袋。3组分别在保存时间为1，3及5d时取样，检测乳酸、乳酸脱氢酶（LDH）、葡萄糖含量和pH值等代谢功能相关指标，并进行统计学分析。结果随着保存时间延长，单采血小板悬液的pH值及葡萄糖含量呈逐渐下降趋势，差异有统计学意义（P〈0．05）；而LDH及乳酸含量呈逐渐升高趋势，差异亦有统计学意义（P〈0．05）。结论单采血小板悬液在22℃保存5d内，其代谢功能发生明显变化。临床输注的单采血小板在储存时间内，其储存时间越短，质量越好。Objective To study the effect of storage time on metabolic function of apheresis platelets. Methods Selected a total of 30 bags of apheresis platelets which were collected from 30 cases of voluntary blood donors as research subjects. The volume of each bag of apheresis platelets was one therapeutic dose, and platelet count was （2. 543. 0）×10^11/L. All of 30 bags of apheresis platelets would been stored in storage tank for 5 days at 22 ℃. The 30 bags of apheresis platelets were randomly divided into storage for 1 day group （n=10）, storage for 3 days group and storage for 5 days group （n=10） equally by digital table method after being numbered. Content of lactic acid, lactate dehydrogenase （LDH）, content of glucose and pH value were detected among 3 groups. Relationship of the metabolic function of apheresis platelets and storage time were analyzed by statistical method. Results The longer the storage duration time of apheresis platelets was, the lower of pH values and content of glucose in apheresis platelets were, and there were significant differences among 3 groups （P^0.05）. At the same time, contents of LDH and lactic acid in apheresis platelets were gradually increased, and there also were significant differences among 3 groups （P〈0.05）. Conclusions There are marked changes for metabolic function of apheresis platelets in storage of 5 days at 22℃. The shorter the storage duration time of apheresis platelets is, the better quality of apheresis platelets is.

关 键 词：血液成分输血 血小板输注 单采血小板 代谢 血小板功能试验 血液保存 临床输血 

分 类 号：R457.1[医药卫生—治疗学]