杨梅黄酮对炎性痛大鼠的外周镇痛作用  

Periphery analgesic effect of myricetin on a rat model of inflammatory pain

作　　者：宋英[1] 张咏梅[1] 徐洁[1] 花嵘[2] 

机构地区：[1]徐州医学院麻醉学重点实验室,江苏徐州221004 [2]中国人民解放军第九七医院急诊科,江苏徐州221000

出　　处：《中国药理学通报》2015年第8期1108-1111,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年项目(No 31200836);徐州医学院振兴计划资助项目;院长专项人才基金(No 2012KJZ15);青蓝工程资助

摘　　要：目的研究杨梅黄酮对炎性痛大鼠的外周镇痛作用及机制。方法采用大鼠左侧后肢足底中心皮下注射完全弗氏佐剂(complete Freund's adjuvant,CFA)建立慢性炎性痛模型。检测给予杨梅黄酮前后大鼠热缩足反射潜伏期(thermal withdrawal latency,TWL)的改变。电生理学方法分析杨梅黄酮对背根节神经元放电频率及电压依赖性钾电流的影响。结果大鼠左侧足底皮下注射CFA致炎后,TWL明显降低(P<0.05),腹腔注射50、500 mg·kg-1杨梅黄酮使CFA组大鼠TWL明显升高(P<0.05),电流钳记录显示杨梅黄酮抑制背根节小神经元的放电频率(P<0.01),且该抑制作用是通过增加钙依赖性钾通道电流(P<0.05)产生的。结论杨梅黄酮通过增加背根节神经元钙依赖性钾电流,抑制神经元兴奋性而发挥外周镇痛作用。Aim To investigate the periphery analge- sic effect of myricetin on a rat model of inflammatory pain and the mechanism. Methods Rat models of in- flammatory pain were induced by complete Freund＇ s adjuvant （CFA） injection in left hindlimb plantar cen- ter. The thermal withdrawal latency （TWL） was meas- ured before and after CFA or myricetin treatment. Elec- trophysiological method was used to identify the effect of myricetin on the action potential frequency and the voltage dependent potassium channel currents in small DRG neurons. Results Rats with CFA injected showed thermal hyperalgesia （ P 〈 0. 05 ） and TWL in- creased significantly after myricetin intraperitoneally in-jected （ P 〈0. 05 ）. Current clamp recording showed the action potential frequency of small DRG neurons in rats was inhibited by myrieetin （ P 〈 0.01 ） and voltage calmap recording showed the inhibitory effect of myr- icetin was enhanced by calcium depended potassium channel currents （ P 〈 0. 05 ）. Conclusion Myricetin exerts periphery analgesic effect by enhancing calcium depended potassium channel currents and inhibiting excitability of small neurons of dorsal root ganglion .

关 键 词：杨梅黄酮 完全弗氏佐剂 钾电流 炎性痛 背根神经节 膜片钳 

分 类 号：R-332[医药卫生] R284.1