白术挥发油的质量标准研究  

Study on Quality Standards of Volatile Oil of Rhizoma Atractylodis Macrocephalae

作　　者：孙林芳[1] 朱莹[1] 贺嵩敏[1] 冯兵[1] 郑广娟[1] 牟洁 

机构地区：[1]广东省中医院 [2]山东医学高等专科学校,山东济南250000

出　　处：《山东中医药大学学报》2015年第4期372-376,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省科技厅重大项目(编号:2008GG30002027)

摘　　要：目的:建立白术挥发油的质量控制方法 ,为新剂型的研发奠定基础。方法 :采用Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm)色谱柱;流动相:乙腈(A)-水(B),梯度洗脱(0~8 min,40%B^28%B;8~11.5 min,28%B^24%B;11.5~20 min,24%B^21%B;20~25 min,21%B^5%B;25~35 min,5%B^0%B;35~40 min,0%B);流速1.0 m L·min-1;含量测定波长220 nm;指纹图谱检测波长200 nm;柱温30℃。结果:挥发油的HPLC含量测定和指纹图谱分析方法学考察结果良好。15批挥发油的指纹图谱与共有模式之间的相似度在0.981~1.000之间,15批挥发油特征图谱及共有模式中的特征峰峰面积占总峰面积的91.175%~96.628%,平均百分比为94.49%。结论 :建立的HPLC挥发油含量测定及指纹图谱分析方法简单、稳定、可靠,15批挥发油指纹图谱整体相似度较高,特征成分种类相近,但其相对含量有差异。Objective：To establish a quality control system of the volatile oil of atractylodes,lay a foundation for new agent research and development. Methods：The methods were carried out by Agilent ZORBAX SB-C18（4.6 mm×250 mm,5 m） column,using acetonitrile（A）-water（B） as mobile phase with gradient elution（0~8 min,40% B^28% B;8~11.5 min,28% B^24% B;11.5~20 min,24% B^21% B;20~25 min,21% B^5% B;25~35 min,5% B^0% B;35~40 min,0% B）,at a flow rate of 1.0 m L·min-1. The quantitative quality control detection wavelength was set at 220 nm. The specific chromatograms detection wavelength was set at 200 nm.The column temperature was 30 ℃. Results：The HPLC methods of quantitative quality control and specific chromatograms for the volatile oil were established. The sum of the specific peaks area in feature chromatograms and mutual mode of fifteen batches volatile oil accounted for 91.175% ~96.628% of the total peaks area,the average percentage was 94.49%. Conclusion：The established methods of quantitative quality control and specific chromatography for the volatile oil by HPLC were simple,accurate and reliable. The total similarity degree of the feature chromatograms gained in fifteen batches volatile oil were higher and the varieties of their feature components were very closely same,but the relative content of these components had some difference.

关 键 词：白术挥发油 质量标准 指纹图谱 高效液相色谱 白术内酯Ⅰ 白术内酯Ⅲ 

分 类 号：R284.1[医药卫生—中药学]