复方蒿甲醚对伯氏疟原虫青蒿素抗性鼠疟疗效的实验研究  

Efficacy of artemether-lumefantrine( Coartem) in treatment of artemisinin-resistant Plasmodium berghei malaria in mice

作　　者：孙雪英[1] 代勇[1] 徐志慧[1] 韩韬[1] 梁昊[1] 孙红霞[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《湖南中医杂志》2015年第7期157-159,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：四川省科技支撑计划项目(编号:2011SZ0315);四川省科技支撑计划成果转化专项项目(编号:2011CSZ0021);国家"重大新药创制"科技重大专项"专利到期药再创新"项目(编号:2012ZX09202101-005)

摘　　要：目的:探讨复方蒿甲醚对伯氏疟原虫青蒿素抗性鼠疟的疗效。方法:伯氏疟原虫常规株经青蒿素逐量递增给药法连续培养16代,建立伯氏疟原虫青蒿素抗性株鼠疟模型。抗疟药效实验采用4d抑制给药法,采血镜检药后24h原虫血症密度、转阴率及28d治愈率。结果:经青蒿素逐量递增给药法连续培养16代后伯氏疟原虫青蒿素抗性株ED50为839.46mg/kg,抗性指数为3.33。青蒿素抗性鼠疟对本芴醇有可疑耐药(原虫血症密度>0.005);复方蒿甲醚对青蒿素抗性鼠疟药后24h的疗效优于本芴醇。复方蒿甲醚及蒿甲醚对青蒿素抗性鼠疟28d治愈率分别为70%和100%。青蒿素、本芴醇和磷酸氯喹分别对青蒿素抗性鼠疟有不同程度转阴,但都出现复燃,均未实现28d治愈。结论:青蒿素抗性鼠疟对本芴醇可疑耐药,可能是复方蒿甲醚对此抗性疟治疗后复燃的因素之一。Objective ： To investigate the efficacy of artemether - lumefantrine （AL） in the treatment of artemisi- nin -resistant Plasmodium berghei malaria in mice. Methods：A conventional Plasmodium berghei strain was subjec- ted to increasing concentration of artemisinin for 16 serial passages and a mouse model of artemisinin - resistant Plasmodium berghei malaria was established. The efficacy of antimalarial drugs was determined by the standard 4 - day suppressive test. Parasitemia level and clearance rate at 24 hours after treatment and 28 - day cure rate were de- termined by microscopic examination of the blood samples collected. Results：After 16 serial passages with increas- ing concentration of artemisinin, the effective dose that reduced artemisinin - resistant Plasmodium berghei by 50% （ ED50 ） was 839. 46mg/kg and the resistance index was 3.33. Artemisinin - resistant malaria in mice had suspicious resistance to benflumetol （parasitemia level 〉 0. 005）. The efficacy of AL at 24 hours after treatment was superior to that of benflumetol in treatment of artemisinin - resistant malaria in mice. The 28 - day cure rates of AL and ar- temether for artemisinin -resistant malaria in mice were 70% and 100%, respectively. For artemisinin -resistant ma- laria in mice, artemisinin, benflumetol, and chloroquine phosphate achieved different clearance rates;however, all of them failed to prevent relapse and cure malaria within 28 days. Conclusion ：Artemisinin -resistant malaria in mice has suspicious resistance to benflumetol,which might be one of the factors for the relapse after treatment with AL.

关 键 词：青蒿素抗性鼠疟 复方蒿甲醚 伯氏疟原虫 实验研究 

分 类 号：R285.5[医药卫生—中药学]