健脾益肠散对溃疡性结肠炎大鼠IFN-γ、IL-10、TGF-β1的影响  

Effect of Jianpi Yichang Powder on IFN- γ,IL-10 and TGF-β1 in Ulcerative Colitis Rats

作　　者：刘金国[1] 蔺晓源[2] 张文秀[3] 张瑜[1] 纪云西[4] 刘杰民[5] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]湖南中医药大学第一附属医院,湖南长沙410007 [3]贵阳医学院,贵州贵阳550004 [4]广西中医药大学第一附属医院,广西南宁530023 [5]贵州省人民医院,贵州贵阳550002

出　　处：《中医药信息》2015年第4期7-9,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81160441)

摘　　要：目的:观察健脾益肠散对溃疡性结肠炎(UC)大鼠血清IFN-γ、IL-10、TGF-β1的影响。方法:将60只SD大鼠按体质量随机分为正常组、模型组、健脾益肠散高、中、低剂量组和柳氮磺吡啶组,每组10只;采用2、4、6-三硝基苯磺酸(TNBS)/乙醇法复制UC大鼠模型;给药21天后,ELISA法测定各组大鼠血清IFN-γ、IL-10、TGF-β1含量。结果:模型组大鼠血清IFN-γ、TGF-β1含量增加,IL-10含量减少,与正常组比较均有统计学差异(P<0.01);与模型组比较,健脾益肠散高、中剂量组IFN-γ、IL-10含量和高剂量组TGF-β1含量均明显改善(P<0.05或P<0.01);健脾益肠散高剂量组血清IFN-γ、IL-10、TGF-β1的变化与柳氮磺吡啶组比较差异无统计学意义(P>0.05),但其明显优于健脾益肠散低剂量组(P<0.05)。结论:健脾益肠散对UC肠黏膜免疫的保护作用可能与调节外周血IFN-γ、IL-10、TGF-β1的含量有关。Objective：To observe the effect of Jianpi Yichang powder（ JP） on IFN-γ,IL-10 and TGF-β1 in ulcerative colitis（ UC） rats.Methods：60 SD rats were randomly divided into a normal group,a model group,JP high,medium and low-dose groups,a sulfasalazine group by body quality,with 10 in each group.Use 2,4, 6-trinitrobenzene sulfonic acid （ TNBS） /ethanol method to replicate UC rat models.The levels of serum I FN-γ,IL-10 and TGF-β1 were detected by ELISA in each group after gavage for 21days.Results：The ser-um contents of IFN-γand TGF-β1 increased,but IL-10 decreased in model group.Compared with the normal group,the difference was statistically significant（ P＜0.01） .IFN-γand IL-10 contents in JP high, middle dose groups and the TGF-β1 in high dose group were significantly improved compared with those in the model group（P＜0.05 or P＜0.01）.In the JP high-dose group, the differences of IFN-γ,IL-10 and TGF-β1 with those in the sulfasalazine group were not significant（P＞0.05）,but it was significantly better than the JP low-dose group（P＜0.05）.Conclusion： Protective effects of JP on intestinal mucosal immunity of UC may be related to adjusting the contents of IFN-γ,IL-10 and TGF-β1 in peripheral blood.

关 键 词：溃疡性结肠炎 健脾益肠散 IFN-Γ IL-10 TGF-Β1 

分 类 号：R285.5[医药卫生—中药学]