舒芬太尼后处理对大鼠心肌缺血再灌注损伤的保护作用  

The protective effect of sufentanil postconditioning on myocardial ischemia-reperfusion injury in rats

作　　者：张燕[1] 吴新海[1] 黄飞[1] 徐向辉[1] 张晓晖[2] 

机构地区：[1]北京大学深圳医院麻醉科,深圳市518036 [2]深圳市中医院中医药研究所,518000

出　　处：《实用医学杂志》2015年第15期2440-2442,共3页The Journal of Practical Medicine

基　　金：深圳市科创委基金资助(编号:JCYJ20130402114317403)

摘　　要：目的:评价舒芬太尼后处理对大鼠心肌缺血再灌注损伤是否具有保护作用,并探讨其可能的机制。方法:雄性SD大鼠30只,随机分为5组(n=6):假手术组(S组)、缺血再灌注组(I/R组)、舒芬太尼低剂量组(SL组)、舒芬太尼高剂量组(SH组)和舒芬太尼+渥曼青霉素组(WM组)。于再灌注前,各组分别注入以下药物:SL组予生理盐水及舒芬太尼1μg/kg,SH组予生理盐水及舒芬太尼3μg/kg,WM组予渥曼青霉素15μg/kg及舒芬太尼1μg/kg,S组及I/R组予生理盐水。于再灌注末,采集动脉血检测血浆c Tn I浓度;取心脏测定缺血危险区(AAR)和梗死区(IS)体积。结果:与S组相比,其余四组c Tn I浓度均明显升高(P<0.01);与I/R组比较,SL组、SH组c Tn I浓度及IS/AAR均明显降低(P<0.01),WM组c Tn I浓度明显降低(P<0.01);与SL组比较,SH组c Tn I浓度及IS/AAR均明显降低(P<0.01),WM组均明显升高(P<0.01)。结论:舒芬太尼后处理可减轻大鼠心肌缺血再灌注损伤,该保护作用与PI3K相关信号通路的激活有关。Objective To evaluate the effect of sufentanil postconditioning on myocardial ischemia- reperfusion injury in rats, and to investigate the possible mechanism. Methods Thirty male Sprague-Dawley ratswere randomly divided into 5 groups （n = 6 each）： sham group （group S）, I/R group （group I/R）, low dose of sufentanil postconditioning group （group SL）, high dose of sufentanil postconditioning group （group SH） andsufentanil postconditioning plus wortmannin group （group WM）. Different drugs were injected before reperfusion： notmal saline （NS） and sufentanil 1 μg/kg in group SL, NS and sufentanil 3 μg/kg in group SH, wortmannin 15 μg/kg andsufentanil 1 μg/kgin group WM, and NS in group S and I/R. At the end of reperfusion, artery blood was collected for assessment of plasma cTnI concentration; And the heart was harvested for determination of infarct size （IS） and area at risk （AAR）. Results Compared with group S, cTnI concentration was inereasedin the rest groups （P〈 0.01 ）; Compared with group I/R, cTnI concentration was decreased in group SL, SH and WM, while IS/AAR reduced in group SL and SH （P 〈 0.01 ） ; Compared with group SL, cTnI concentration and IS/AAR were decreased in group SH while increased in group WM （P 〈 0.01 ）. Conclusion Sufentanil postconditioning could attenuate myocardial ischcmia-reperfusion injury in rats, and the mechanism involved PI3K related pathway.

关 键 词：舒芬太尼 药物后处理 心肌缺血再灌注损伤 PI3K 

分 类 号：R542.2[医药卫生—心血管疾病]