肠系膜淋巴管结扎对重症急性胰腺炎大鼠肝损伤的影响  

Effect of mesenteric lymphatic ligation on liver injury induced by severe acute pancreatitis in rats

作　　者：刘媛琪[1] 王璐璐[1] 陈立[1] 谭晓勇[1] 范玲[1] 熊玉霞[1] 

机构地区：[1]泸州医学院药学院药理教研室,646000

出　　处：《实用医学杂志》2015年第14期2252-2254,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81102868)

摘　　要：目的:观察肠系膜淋巴管结扎对重症急性胰腺炎致大鼠肝损伤的影响。方法:雄性SD大鼠54只,随机分为假手术组(A)、重症急性胰腺炎组(B)和重症急性胰腺炎+肠系膜淋巴管结扎组(C),分别于术后6、12和24 h采血及大鼠肝组织,HE染色观察肝脏病理组织学改变,血生化检测血清丙氨酸转氨酶(alanine transaminase,ALT)及天门氨酸氨基转移酶(aspartate transaminase,AST)水平,ELISA法测定肝组织匀浆肿瘤坏死因子-α(tumor necrotic factor-α,TNF-α)及白细胞介素-1(interleukin-1,IL-1)水平。结果:光镜下,B组大鼠肝小叶中性粒细胞及炎性渗出物随着时间推移逐渐增多,C组大鼠炎症较B组有所减轻;各时间点B组和C组ALT和AST均显著高于A组(P<0.05),C组显著低于B组(P<0.05);B组大鼠TNF-α和IL-1水平在3个时间点均显著高于A组(P<0.05),C组24h TNF-α、IL-1水平均显著低于B组(P<0.05)。结论:肠系膜淋巴管的结扎可减轻重症急性胰腺炎所致的肝损伤,提示肠系膜淋巴液可能在重症急性胰腺炎所致的肝损伤中发挥着重要作用。Objective To explore the effect of mesenteric lymphatic ligation on liver injury induced by severe acute pancreatitis. Methods 54 SD rats are randomly divided into sham-operation group （A）, severe acute pancreatitis group （B） and severe acute pancreatitis and ligation group （C）. Liver tissues were collected from each rat in 6, 12, and 24 h after ligation. Pathological changes in liver tissues were observed by haematoxylin and eosin staining. Levels of serum glutamic-pyruvic transaminase and glutamic oxalaeetic transaminase were detected. Levels of TNF-α and IL-1 in liver homogenate were examined by enzyme linked immunosorbent assay. Results Under light mieroseopie examination, neutrophils and inflammatory exudate in hepatic lobule was increased as time prolonged in group B. Inflammation was reduced in group C as compared with group B.Levels of serum glutamie-pyruvie transaminase and glutamie oxalaeetie transaminase were more significantly increased in groups B and C than in group A （P 〈 0.05） ; while they were more significantly decreased in group C than in group B. Levels of TNF-α and IL-1 in liver homogenate were significantly increased in group B as compared with group A （P 〈 0.05）; whereas they were more significantly decreased in group C than in group B at 24 h （P 〈 0.05）. Conclusions Mesenterie lymphatic ligation can reduce liver injury in severe acute pancreatitis. Mesenterie lymphatie fluids may play an important role in severe aeute pancreatitis.

关 键 词：肠系膜淋巴管 结扎 重症急性胰腺炎 肝损伤 

分 类 号：R657.51[医药卫生—外科学]