GDF-15在慢性高原病大鼠模型中的表达及其与hepcidind的关系  

Expression of GDF-15 in chronic mountain sickness rat model and discussion of the relationship with GDF-15 and hepcidin

作　　者：耿惠[1] 陈碧琴[1] 

机构地区：[1]青海大学附属医院,西宁市810001

出　　处：《实用医学杂志》2015年第14期2255-2257,共3页The Journal of Practical Medicine

基　　金：2013年青海大学中青年科研基金(编号:2013-QYY-1);2013年青海大学附属医院中青年科研基金(编号:ASRF-2013-08)

摘　　要：目的:研究慢性高原病(CMS)大鼠骨髓GDF-15基因及蛋白的改变,探讨GDF-15在CMS发病机制中的作用及其与hepcidin之间的关系。方法:CMS大鼠模型32例作为实验组,西宁地区饲养的常氧大鼠16例作为对照组,检测骨髓GDF-15、骨髓单个核细胞GDF-15 mRNA、血清EPO及hepcidin水平、肝脏铁调素基因(HAMP)mRNA的表达。结果:实验组骨髓GDF-15蛋白及mRNA表达明显高于对照组(P<0.01);实验组EPO水平明显高于对照组(P<0.01);实验组骨髓GDF-15与血清EPO呈正相关(r=0.397,P=0.031)、与血清hepcidin无相关性(r=-0.224,P=0.218)。结论 :GDF-15促进了CMS红系生成,其水平的高低可反映CMS红系的增生情况;GDF-15对HAMP表达无抑制作用。Objective To determine the growth differential factor 15 （GDF-15） in CMS rat model, investigate the significance of GDF-15 in CMS and the relationship between GDF-15 and hepeidin. Methods 32 rats of CMS model were taken as experimental group （EXP）, the other 16 rats fed in Xining （CON） were taken as control group. The mRNA and protein expression levels of GDF-15 were detected respectively. Results Compared with that in CON group, the level of mRNA and protein of GDF-15 were significantly higher in EXP group （P 〈 0.01 ）. GDF-15 and EPO had correlation in EXP （r = 0.397, P = 0.031）, but had no correlation with serum hepcidin in EXP （r = -0.224, P = 0.218）. Conclusion GDF-15 can promote CMS and represent erythrocytosis, while GDF- 15 has no inhibition to the expression of hepeidin.

关 键 词：慢性高原病 低氧 GDF-15 红细胞生成 

分 类 号：R594.3[医药卫生—内科学]