连瓣兰组织培养及菌根化研究  

Study on Tissue Culture and Mycorrhizal Fungi of Cymbidium lianpan

作　　者：段雪甜 陈雨薇[1,2] 王芳[1] 李婷婷[2] 伍建榕[1,2] 

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 [2]西南林业大学林学院,云南省高校森林灾害预警控制重点实验室,云南昆明650224

出　　处：《北方园艺》2015年第15期111-113,共3页Northern Horticulture

基　　金：国家自然科学基金资助项目(31360198;31260175);云南省教育厅科学研究基金资助项目(2013Y123);云南省高校干热河谷植被恢复创新团队资助项目;云南省重点学科森林保护学资助项目(XKZ200905);云南省高等学校森林病虫害综合治理教学团队资助项目(20126005)

摘　　要：以野生连瓣兰和组培连瓣兰为试材,采用内生真菌分离鉴定、内生真菌与连瓣兰组培苗的共生培养以及制作石蜡切片对其菌根结构进行观察等方法,研究菌根真菌对连瓣兰生长的影响,并找出适合连瓣兰生长的菌根真菌。结果表明:从保山野外采集的12株连瓣兰中分离得到的10个菌株中,有3株适合与组培苗进行共生培养,分别是丝核菌属(Rhizoctoniasp.)、毛壳菌属(Chaetomiumsp.)、镰刀菌属(Fusariumsp.),其中丝核菌效果最佳,对无菌组培苗生长有良好的促进作用并在无菌组培苗根部形成菌丝结。Using wild Cymbidium lianpan and Cymbidium seed as test materials,using the method of isolating and identificating endophytic fungus,examining the interaction between aseptic seedlings and endophytic fungus in Cymbidium lianpan and making paraffin section of the mycorrhizal structure observation,to study the effect of mycorrhiza fungi on cymbidium lianpan growth,and find out the suitable mycorrhizal fungi for growth of Cymbidium.The results showed that from Baoshan field collection 12 cymbidium lianpan of 10 strains of isolated Cymbidium,there were three suits with the somaclone symbiotic cultivation,respectively was Rhizoctoniasp.,Chaetomiumsp.,Fusarium sp.,among them,the effect of Rhizoctoniasp.was the best,had a good role in promoting growth of sterile somaclone hypha and sterile somaclone in the root formation.

关 键 词：连瓣兰 组织培养 菌根真菌 共生培养 菌根化 

分 类 号：S682.31[农业科学—观赏园艺]