利用SSR标记分析玉米P-162近缘系的遗传多样性  

Genetic Diversity Analysis of Maize P-162 Related Inbred Lines by SSR Markers

作　　者：彭富海 龙声卫 郭仲举 李源念 

机构地区：[1]贵州黔西南喀斯特区域发展研究院,贵州兴义562400

出　　处：《玉米科学》2015年第4期33-37,共5页Journal of Maize Sciences

基　　金："贵州省玉米产业技术体系-黔西南州综合实验站"(GZCYTX2013-07004);"青贮杂交玉米盘江7号示范应用"(黔科合农字[2013]5087号);"饲料玉米盘江7号种植青贮与养羊技术示范推广应用"(黔科合农字[2013]11号);"贵州山区玉米小麦等抗旱关键技术集成与示范"(2012BAD40B03-1);"黔西南州主要粮食经作物抗旱关键技术研究与示范"(黔科合NY[2010]3081号)

摘　　要：利用SSR标记技术分析47个玉米P-162近缘系的亲缘关系及遗传多样性,研究P-162自交系的亲缘关系。结果表明,22对多态性较好的随机引物中共检测到154个等基因变异,每对引物检测到等位基因3~12个,平均7.48个。利用UPGMA聚类分析法将供试自交系划分为7类,47个玉米P-162近缘系材料之间具有一定的遗传差异,总体供试材料遗传基础相对较狭窄。In order to study the genetic relationship of P- 162 inbred lines, the genetic relationship and genetic diversity of forty-seven P- 162 inbred lines were analyzed by using SSR markers. The results showed that twenty- two pairs of high polymorphic random primers detected 154 gene mutations, there were 3-12 alleles in each pair of primers, about 7.48 on average. By using UPGMA cluster analysis method, the tested inbred lines were divided into 7 categories, forty-seven P-162 related lines had some genetic difference between the departments of materials, but the overall test material was relatively narrow genetic basis.

关 键 词：玉米 P-162近缘系 遗传多样性 SSR标记 

分 类 号：S513.035.3[农业科学—作物学]