玉米内生细菌-解淀粉芽孢杆菌Y19-RifM在玉米根部定殖能力的研究  

Colonization of Endophytic Bacillus amyloliquefaciens Y19-RifM in Corn Rhizosphere

作　　者：郭力维[1] 吴毅歆[2,3] 何月秋[1,2,3] 

机构地区：[1]云南农业大学,农业生物多样性应用技术国家工程中心,昆明650201 [2]云南农业大学农学与生物技术学院,昆明650201 [3]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650207

出　　处：《玉米科学》2015年第4期132-137,共6页Journal of Maize Sciences

基　　金：国家“863”计划项目(2011AA10A205);云南省农业产业技术体系项目

摘　　要：采用逐步提高LB平板中利福平浓度的方法,从具有促生长和防病潜力的玉米内生细菌解淀粉芽孢杆菌Y19中筛选出获得抑菌活性不变的抗利福平（250μg/m L）突变菌株Y19-Rif M,研究Y19-Rif M在玉米根部定殖能力。结果表明,玉米出苗后25、30、35、40、45、50、55 d,从根际、根表、根内均能回收到抗性菌落。根际、根表的回收菌量保持在1×10^3~1×10^4CFU/g的水平,根内保持在1×10^3CFU/g的水平,灭菌土中回收到的菌落数量高于自然土中菌落数量。Colonization ability of the endophytic Bacillus amyloliquefaciens YI9 with good abilities to promote plant growth and inhibit fungal pathogens was studied by a rifampicin（250 μg/mL） resistant strain Y 19-RifM ob- tained from LB plates with a series of increasing antibiotic concentrations. Y19-RifM could be recovered from rhi- zospere soil, root surface and inner root tissue in 25, 30, 35, 40, 45, 50 and 55 days after corn emergency in the greenhouse. The results showed that the colony number reached up to 1× 10^3 - 1 × 10^4 CFU/g from corn rhizosphere and root surface and 1 ×10^3 CFU/g from inner root tissue. The colony number for the corn growing in sterilized soil was higher than that in natural soil.

关 键 词：玉米 玉米内生细菌 解淀粉芽孢杆菌Y19 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]