东北地区玉米茎腐病镰孢菌EF-1α基因序列分析鉴定  

Sequence Analysis of EF-1α Gene in Fusarium Species Causing the Maize Stalk Rot in Northeast China

作　　者：陈楠[1] 潘晓静[1] 姚远[1] 刘博[1] 刘限[1] 高增贵[1] 

机构地区：[1]沈阳农业大学植物免疫研究所,沈阳110866

出　　处：《玉米科学》2015年第4期143-148,共6页Journal of Maize Sciences

基　　金：国家科技支撑项目(2012BAD19B04)

摘　　要：从东北地区采集玉米茎腐病标样90份,经分离纯化获得镰孢菌120株,在传统形态学鉴定的基础上,选取21株具有代表性菌株,采用基因组DNA的EF-1α序列分析技术进行镰孢菌种类的辅助鉴定。结果表明,引起玉米茎腐病的主要致病菌有4种,分别是轮枝镰孢菌(Fusarium verticillioides)、禾谷镰孢菌(Fusarium graminearum)、胶孢镰孢菌(Fusarium subglutinans)和层生镰孢菌(Fusarium proliferatum),分离频率分别为45.45%、36.36%、15.15%、3.03%。A total of 120 Fusarium were obtained from 90 diseased plants of maize stalk rot in Northeast China, on the basic of traditional morphological classification, typical strains of 21were selected. Using EF- lα gene se- quence analysis methods, four Fusarium species, Fusarium verticillioides, Fusarium graminearum, Fusarium subglu- tinans, Fusarium proliferatum were identified. Separation frequency was 45.45%, 36.36%. 15.15% and 3.03%_

关 键 词：玉米 玉米茎腐病 镰孢菌 EF-1α序列 种类鉴定 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]