禽致病性大肠杆菌脂多糖核心型分布与毒力基因的相关性分析  

Distribution of lipopolysaccharide core type in avian pathogenic Escherichia coli and its correlation with virulence gene

作　　者：张宇曦[1,2] 韩先干[2] 左佳坤 龚建森[3] 韩月[2] 范国博 王少辉[2] 田明星 丁铲[2] 祁克宗[1] 于圣青[2] 

机构地区：[1]安徽农业大学,安徽合肥230036 [2]中国农业科学院上海兽医研究所,上海200241 [3]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《微生物学通报》2015年第8期1619-1625,共7页Microbiology China

基　　金：国家自然科学基金项目(No.31370045;31372402);上海市科技兴农重点攻关项目[No.泸农科攻字(2015)第1-9号];农业公益性行业科研专项项目(No.201303044)

摘　　要：【目的】大肠杆菌脂多糖(LPS)核心型根据其化学结构的不同分为5种,即R1、R2、R3、R4和K12。通过对禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)安徽、江苏、上海和河南等省市分离株的脂多糖核心型分布情况的研究,分析其与大肠杆菌主要毒力基因之间的潜在联系,以期为APEC的研究和防治提供参考。【方法】对分离到的76株APEC,利用PCR方法开展对LPS核心型分型鉴定和毒力基因检测;分析LPS核心型的分布和毒力基因、致病性之间的相关性。【结果】在76株APEC分离株中,68.4%(52株)为R1核心型,15.8%(12株)为R3型,11.8%(9株)为R4型,3.9%(3株)为R2型,未检测到K12核心型。毒力基因鉴定结果中yijp、mat、fim C、ibe B和omp A的检验阳性率均达到90%以上,可作为APEC的保守基因。其中LPS核心型R1与neu C、cva/cvi、irp2均具有显著正相关性(P<0.05),R3与iro N、irp2均具有显著负相关性(P<0.05),R4核心型与aat A显著正相关(P<0.05)。【结论】APEC的LPS核心型主要为R1。LPS核心型对部分毒力基因分布具有显著影响。[Objective] The core type of Escherichia coli lipopolysaccharide（LPS） is divided into 5 types, R1, R2, R3, R4 and K12, based on their different chemical structure. The aim of the present study was to study the distribution of LPS core type in avian pathogenic E. coli, and its correlation with virulence genes. [Methods] LPS core type and 17 virulence genes were detected in 76 strains of APEC by PCR. The correlation of LPS core type and virulence was analyzed. [Results] The core types of R1, R3, R4 and R2 were 68.4%, 15.8%, 11.8% and 3.9%, respectively. The virulence genes yijp, mat, fim C, ibe B and omp A were conserved in APEC strains. The R1 core type was significantly positive correlated with neu C, cva/cvi and irp2（P0.05）, the R4 core type was positive correlated with aat A, whereas the R3 core type was negative correlated with iro N and irp2（P0.05）. [Conclusion] R1 core type is more prevalent than other LPS core types and associated with high pathogenicity.

关 键 词：禽致病性大肠杆菌 脂多糖核心型 毒力基因 分布 致病性 相关性分析 

分 类 号：S852.61[农业科学—基础兽医学]