盐胁迫对甘草愈伤组织渗透调节的影响  

Effects of Salt Stress on Osmotic Regulation of Glycyrrhiza uralensis Callus

作　　者：柳福智[1] 羊健麟 

机构地区：[1]甘肃农业大学农学院,兰州730070

出　　处：《植物生理学报》2015年第7期1038-1044,共7页Plant Physiology Journal

基　　金：甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1429)

摘　　要：研究了盐胁迫对甘草不同外植体叶和下胚轴愈伤组织生长速率及几种渗透调节物质累积量的影响。结果表明:在盐胁迫条件下,甘草叶子和下胚轴愈伤组织的相对生长率都随着盐浓度的增加先升高后降低。甘草愈伤组织具有很强的耐盐能力,低于85.5 mmol·L-1 Na Cl处理可明显促进愈伤组织的生长,高于85.5 mmol·L-1 Na Cl处理,促进作用减弱,愈伤组织生长呈下降趋势。当Na Cl浓度高于136.8 mmol·L-1时,甘草愈伤组织中无机盐离子Cl-和Na+大量积累,使K+外渗,从而使K+/Na+大幅度下降。同时,甘草愈伤组织中脯氨酸、可溶性糖、甜菜碱等在盐胁迫下也大量累积。这些结果表明一定程度盐胁迫下甘草通过积累无机离子和可溶性小分子物质进行渗透调节,是甘草耐盐性强的重要原因之一。An experiment was conducted to study the effects of salt stress on callus induced by leaf and hypocotyl of licorice growth rate and several cumulative amount of osmotic adjustment under the salt stress. The results of this study showed that under the conditions of salt stress, the relative growth rates of callus induced by leaf and hypocotyl of licorice were reduced after the first increased with the increasing of salt concentration. Licorice callus has a strong salt tolerance, lower than 85.5 mmol·L^-1NaCl treatment may promote the growth of callus; higher than 85.5 mmol·L^-1NaCI treatment, it will inhibit the growth of callus; When the concentration of NaCl was higher than 136.8 mmol·L^-1, the inorganic particles of C l and Na^＋ accumulated heavily in licorice callus, this made the K^＋ extravasate, the ratio ofK^＋ and Na^＋ was significant decreased. At the same time, the proline, soluble sugars and betaine in licorice callus also accumulated under salt stress. These results showed that licorice conducted the osmotic adjustment by accumulating the soluble inorganic ions and small molecule substences under certain degree of salt stress, which was one of the important reasons for licorice of strong salt tolerance.

关 键 词：甘草 愈伤组织 盐胁迫 渗透调节 

分 类 号：S567.71[农业科学—中草药栽培]