毛萼香茶菜二萜合酶IeCPS的立体特异性研究  

Analysis on Stereospecifi ty of Diterpene Synthases IeCPS from Isodon eriocalyx

作　　者：杜刚[1,3] 龚海艳[1] 高娟[1] 付小莉[1] 卢山[2] 曾英[1] 

机构地区：[1]中国科学院昆明植物研究所,植物化学与西部植物资源持续利用国家重点实验室,昆明650201 [2]南京大学生命科学学院,南京210093 [3]中国科学院大学,北京100049

出　　处：《植物生理学报》2015年第7期1125-1132,共8页Plant Physiology Journal

基　　金：云南省自然科学基金重点项目(2015FA029);植物分子遗传国家重点实验室

摘　　要：对映-贝壳杉烷型二萜是药用植物香茶菜抗菌抗肿瘤的核心组分,其生物合成的酶分子机制尚未完全明了。前期我们克隆并鉴定了毛萼香茶菜二萜合酶柯巴基焦磷酸合酶基因Ie CPS1和Ie CPS2。本文通过与拟南芥对映-贝壳杉烯二萜合酶At KS配对进行偶联反应,以及GC-MS鉴定酶联反应产物,证实毛萼香茶菜二萜合酶Ie CPS1和Ie CPS2的立体特异性为对映型(ent-CPS)。Ent-kaurane diterpenoids, isolated from the Chinese medicinal herbs, Isodon L., are principle components showing potent bioactivities of antitumor and anti-autoimmune inflammation. Despite a large number of ent-kaurane diterpenoids isolated from lsodon plants, little is known about the enzymatic machinery involved in their biosynthesis. IeCPS 1 and IeCPS2 encoding copalyl diphosphate synthases （CPS） were previ- ously cloned from the I. eriocalyx leaves and functionally characterized. Here we verified that IeCPS1 and IeCPS2 were ent-CPSs that produced CPP of ent-stereochemistry, by GC-MS analysis of enzymatic products from coupled reactions with a known Arabidopsis ent-kaurene synthase stereospecific for ent-CPP.

关 键 词：二萜合酶 柯巴基焦磷酸合酶 酶偶联反应 毛萼香茶菜 生物合成 

分 类 号：Q946[生物学—植物学]