不同粒径羟基磷灰石粒子对恶性黑色素瘤A375细胞抑制效应的初步研究  

Inhibitory Effect of Hydroxyapatite Particles with Different Size on Malignant Melanoma A375 Cells:A Preliminary Study

作　　者：郭波[1,2] 李波[2] 王滟[3] 洪友良[2] 张伶俐[2] 张兴栋[2] 

机构地区：[1]四川大学华西医院眼科,成都610041 [2]四川大学国家生物医学材料工程技术研究中心,成都610064 [3]四川省人民医院妇产科,成都610072

出　　处：《生物医学工程学杂志》2015年第4期832-837,共6页Journal of Biomedical Engineering

基　　金：国家重点基础研究发展计划"973"计划资助项目(2005CB623901);四川省科技支撑计划资助项目(2010SZ0067)

摘　　要：研究不同粒径的羟基磷灰石(HA)粒子对恶性黑色素瘤A375细胞的抑制效应。湿法合成4种短棒状HA粒子,平均直径分别为998.0nm(HA1)、511.0nm(HA2)、244.0nm(HA3)和71.6nm(HA4),体外干预恶性黑色素瘤A375细胞。结果显示:平均直径小于511.0nm的HA粒子均可抑制A375细胞的增殖,其中纳米级HA4粒子对A375细胞的增殖抑制和诱导凋亡能力最强;HA粒子分布于A375细胞胞质内;HA4组A375细胞超微结构的改变最为显著。研究表明,粒径是HA粒子抗肿瘤效应极为重要的影响因素,纳米级HA4粒子抑制A375细胞增殖的能力最强;纳米HA通过诱导细胞凋亡抑制A375细胞增殖。To explore the inhibitory effect of hydroxyapatite （HA） particles with different sizes on malignant melanoma A375 cells in vitro, we synthesized 4 short rod-like HA particles using TIPS. Their mean diameters were 998.0 nm （HA1）, 511.0 nm （HA2）, 244.0 nm （HA3）, and 71.6 nm （HA4）, respectively. Malignant melanoma A375 cells were co-cultured with HA particles in vitro. Results showed that HA particles smaller than 511.0 nm in mean diameter could always inhibit proliferation of A375 cells, and nanometer-HA particles （HA4） had the strongest inhibitory effect on A375 cell proliferation and the strongest inducing effect on apoptosis. HA particles were distributed in plasma of A375 cells. The uhrastrueture changes of A375 cells were found most significant in nanometer-HA particles （HA4） group. We conclude that particle size is a very important influencing factor on anti-tumor effects of HA and that nanometer-HA particle has the strongest inhibitory effect on tumor cell proliferation.

关 键 词：羟基磷灰石粒子 恶性黑色素瘤细胞 纳米 

分 类 号：R96[医药卫生—药理学]