电针对局灶性脑缺血再灌注模型大鼠学习记忆作用的氧化应激机制  

The possible mechanism of electroacupuncture ameliorating learning and memory ability in rats with focal cerebral ischemia/reperfusion via inhibiting oxidative stress

作　　者：林浴坤 林如辉 陈斌 俞坤强 陶静[1] 

机构地区：[1]福建中医药大学康复医学院,福州350122 [2]福建中西医结合研究院 [3]国家中医药管理局中医康复研究中心 [4]福建康复技术协同创新中心

出　　处：《中国康复医学杂志》2015年第8期755-760,共6页Chinese Journal of Rehabilitation Medicine

基　　金：协同创新中西医结合基础研究实验专项(3021-985010006)(X2012004-协同);国家自然科学基金资助项目(81373778)

摘　　要：目的:通过氧化应激探讨电针神庭、百会对局灶性脑缺血再灌注大鼠学习记忆的影响,阐明其治疗脑缺血后认知功能障碍的可能机制。方法:雄性SD大鼠72只,随机分为假手术组、模型组、电针组、非穴组,每组18只大鼠。以大脑中动脉闭塞(MCAO)法建立大鼠局灶性脑缺血再灌注模型。电针组、非穴组行电针干预7天。各组在造模后3d开始采用Morris水迷宫检测学习记忆能力,免疫组化、免疫蛋白印迹和RT-PCR检测caspase-3表达,用化学比色法检测SOD、MDA和GSH-PX。结果:与其他组相比,电针神庭、百会穴可明显改善MCAO大鼠的神经功能缺损症状;改善MCAO大鼠学习记忆能力;降低皮质caspase-3表达;增强SOD、GSH-PX的活性,降低MDA的含量。结论:电针通过抑制氧化应激、控制神经细胞凋亡,来实现改善局灶性脑缺血再灌注大鼠学习记忆能力,保护神经细胞的损伤。Objective： To investigate the effect of electroacupuncture（EA） ameliorating learning and memory ability in rats with focal cerebral ischemia/reperfusion（I/R） via inhibiting oxidative stress and to clarify the possible mechanism of EA in treatment of cognitive impairment after cerebral ischemia.Method： Seventy-two male adult SD rats were randomly divided into the sham operation control group（SC group）, the ischemia model group（MCAO group）, the electroacupuncture group（EA group） and electroacupuncture non-point group（non-point group）. Each group had 18 rats. Middle cerebral artery occlusion（MCAO） was performed to establish the focal cerebral I/R injury model. The EA group and non-point group received EA for7 d. All rats were tested by Morris water maze to investigate learning and memory ability. Immunohistochemistry, Western blot and RT-PCR were used to test the expression of caspase-3, and using chemical assay to detect SOD, MDA and GSH-PX.Result： Compared with other groups, EA at Shenting（DU24） and Baihui（DU 20） acupoints alleviated neurological deficits significantly and promoted learning and memory ability; reduced caspase-3 expression; enhanced activities of SOD, GSH-PX, reduced the content of MDA.Conclusion：Electroacupuncture can ameliorate learning and memory ability of cerebral I/R injured rats and protect nerve cell damage. Its mechanism may be related to inhibiting oxidative stress and modulating the expression of apoptosis-related gene.

关 键 词：脑缺血 电针 学习记忆 氧化应激 凋亡 

分 类 号：R245[医药卫生—针灸推拿学]