姜黄素激活PPAR-γ通路改变巨噬细胞的极化状态  

Curcumin Changes Macrophage Polarization State by Activating PPAR-γ Pathway

作　　者：桂灵升[1] 李蓉[1] 杨蕾[1] 段凤仪[1] 李岱[1] 张素华[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌内科,重庆400016

出　　处：《中国生物化学与分子生物学报》2015年第8期850-856,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.81200588);重庆市科学技术委员会自然科学基金项目(No.09BB5076);国家临床重点专科建设项目(No.2011)~~

摘　　要：过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorγ,PPAR-γ)通路是调节替换活化的(alternatively activated)M2型巨噬细胞极化的中心环节.姜黄素是PPAR-γ的天然激动剂,有着良好的抗炎作用.本研究通过建立巨噬细胞株的体外炎症模型,用姜黄素及PPAR-γ的特异性抑制剂GW9662对其进行干预,观察巨噬细胞株极化状态的改变.结果显示,姜黄素可以促使巨噬细胞向M2型极化,当特异性抑制PPAR-γ通路后,姜黄素促进巨噬细胞向M2型极化的作用受到抑制.结果表明,姜黄素可能是通过激动PPAR-γ通路促使巨噬细胞向M2型极化,为进一步研究姜黄素的抗炎机制及治疗慢性低度炎症相关的代谢性疾病提供了一个新的思路.Peroxisome proliferator-activated receptor γ（ PPAR-γ） pathway is the central link that regulates alternative activation（ M2） macrophages polarization. Curcumin has a good anti-inflammatory effect as a natural PPAR-γ agonist. This study established a model of macrophage inflammatory in vitro,and intervened with curcumin and specific PPAR-γ inhibitor GW9662 to observe macrophage polarization. The results showed that curcumin promoted M2 macrophages polarization,and this effect was suppressed after inhibiting PPAR-γ. These findings suggested that curcumin might induce M2 macrophage polarization through activating PPAR-γ pathways. It provided a new perspective for the further study of anti-inflammatory mechanism of curcumin and treatment of metabolic diseases associated with chronic low-grade inflammation.

关 键 词：姜黄素 巨噬细胞极化 慢性炎症 代谢性疾病 

分 类 号：R589.2[医药卫生—内分泌]