氯化钙与东莨菪内酯联用对朱砂叶螨的杀螨毒力及其Ca^(2+)-ATP酶活性的影响  

Acaricidal toxicity of scopoletin combined with Ca^(2+) and its influence on Ca^(2+)-ATPase activity in Tetranychus cinnabarinus(Boisduval)

作　　者：侯秋莉 张永强[1,2] 李彩新[1] 丁伟[1,2] 刘雪姣[1] 罗金香[1,2] 

机构地区：[1]西南大学植物保护学院,重庆400716 [2]西南大学农药研究所,重庆400716

出　　处：《农药学学报》2015年第4期475-479,共5页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金项目(31272058);教育部博士点新教师基金项目(20100182120021)

摘　　要：为了明确钙离子与东莨菪内酯联合作用的杀螨效果,进而为东莨菪内酯的开发利用提供参考,采用玻片浸渍法测定了钙离子(Ca2+)与东莨菪内酯混用对朱砂叶螨Tetranychus cinnabarinus雌成螨的杀螨毒力,并测定了活体和离体条件下对螨体内Ca2+-ATP酶活性的影响,对Ca2+的增效作用机理进行了初步分析。结果表明:Ca2+与东莨菪内酯联合使用能显著增强东莨菪内酯的杀螨效果,其中联合作用24和48 h的LC50值分别比东莨菪内酯单独处理降低20%和45%;对朱砂叶螨Ca2+-ATP酶活性的测定结果表明,无论在活体还是离体条件下,Ca2+与东莨菪内酯联用均能显著增强对Ca2+-ATP酶的抑制作用,而相同浓度的Ca2+单独作用则对Ca2+-ATP酶活性无影响,这也在一定程度上证明了Ca2+-ATP酶是东莨菪内酯的重要作用靶标之一。In order to clarify the joint acaricidal action of Ca2＋ and scopoletin,provide theoretical proofs for the development and utilization of scopoletin,the synergism of Ca2＋ to scopoletin on female adult mites,Tetranychus cinnabarinus,were studied by dipping method in the laboratory. The effects of Ca2＋ and scopoletin on Ca2＋ -ATPase activities of T. cinnabarinus were also tested. The results showed that Ca2＋ could dramatically improve the acaricidal activity of scopoletin against T.cinnabarinus,and the LC50 value of scopoletin combined with Ca2＋ were reduced by 20% and 45% at24 h and 48 h,respectively,compared with that of scopoletin used alone. The activities of Ca2＋ -ATPase decreased dramatically after being treated by mixtures of scopoletin and Ca2＋ both in vivo andin vitro,but Ca2＋ had no biological activity alone at the same concentration. The results also suggested that Ca2＋ -ATPase is one of the targets of scopoletin against T. cinnabarinus.

关 键 词：朱砂叶螨 东莨菪内酯 钙离子 杀螨毒力 CA2+-ATP酶 

分 类 号：S482.52[农业科学—农药学]