清毒颗粒抑制免疫逃逸防治大鼠乳腺癌的药效学研究  

Effect of Qingdu Granules on Breast Cancer in Rats via Suppressing Immune Escape

作　　者：陈晨[1] 王秋婷[1] 杨仕军[1] 赵欣[1] 李晓亚[1] 林余霖[1] 蔡大勇[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193

出　　处：《中国药师》2015年第8期1269-1273,共5页China Pharmacist

基　　金：北京自然科学基金项目(编号:7132150)

摘　　要：目的:探讨清毒颗粒对二甲基苯蒽(DMBA)诱导SD大鼠乳腺癌的药效。方法:DMBA诱导大鼠乳腺癌,观察清毒颗粒对肿瘤抑制率,HE染色观察病理特点,IHC检测肿瘤组织Ki-67分布及含量,ELISA检测大鼠血清中IL^-12、IFN-γ、IL-4和IL^-10含量。结果:低、中、高剂量清毒颗粒和柴胡皂苷a的抑瘤率分别为:30.93%,43.84%,44.17%和43.48%;清毒颗粒和柴胡皂苷a可显著降低Ki-67表达量;上调血清中IL^-12、IFN-γ含量,下调IL-4、IL^-10含量。结论:清毒颗粒具有抗乳腺癌作用,其机制可能是调节机体免疫,增强抗肿瘤免疫作用。To study the effect and mechanism of Qingdu granules on the tumor growth of 7, 12-dimethyl-benz[ a] an-thracene （ DMBA）-induced breast cancer in Sprague-Dawley rats. Methods： DMBA was used to induce breast cancer in rats. The tumor inhibition of Qingdu granules was observed. Pathological features were observed after hematoxylin-eosin staining, the distribution and content of Ki-67 in tumor were tested by IHC and the content of IL-12, IFN-γ, IL-4 and IL-10 in serum was determined by ELISA. Results：The inhibitory rate of Qingdu granules at low, middle and high dose and saikosaponin a was 30. 93%,43. 84% and 44. 17% and 43. 48%, respectively. The expression of Ki-67 was reduced in Qingdu granules groups and saikosaponin a group, the content of IL-12 and IFN-γ in serum was increased and the level of IL-4 and IL-10 was reduced in the above groups. Conclusion：Qingdu granules can inhibit breast cancer obviously, and the mechanism is probably related to the ability of immune system adjustment, which can enhance the antitumor effect.

关 键 词：清毒颗粒 抗肿瘤 免疫调节 

分 类 号：R285.5[医药卫生—中药学]