Construction and evaluation of nitric oxide generating vascular graft material loaded with organoselenium catalyst via layer-by-layer self-assembly  

作　　者：AN Jun CHEN SiYuan GAO JingChen ZHANG Xu WANG YuanYuan LI YanDong MIKHALOVSKY Sergey KONG DeLing WANG ShuFang 

机构地区：[1]State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University [2]Institute of Polymer Chemistry, College of Chemistry, Nankai University [3]School of Pharmacy and Biomolecular Sciences, University of Brighton

出　　处：《Science China(Life Sciences)》2015年第8期765-772,共8页中国科学（生命科学英文版）

基　　金：supported by the National Basic Research Program of China(2012CB725204);National Natural Science Foundation of China(51073081,31170030 and J1103503);Program for Changjiang Scholars and Innovative Research Team in University(IRT13023);the Natural Science Foundation of Tianjin,China(13JCYBJC24900 and 13JCZDJC-27800)

摘　　要：A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide(NO) was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione(GSNO). Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts.A new biomimetic material for artificial blood vessel with in situ catalytic generation of nitric oxide （NO） was prepared in this study. Organoselenium immobilized polyethyleneimine as NO donor catalyst and sodium alginate were alternately loaded onto the surface of electrospun polycaprolactone matrix via electrostatic layer-by-layer self-assembly. This material revealed significant NO generation when contacting NO donor S-nitrosoglutathione （GSNO）. Adhesion and spreading of smooth muscle cells were inhibited on this material in the presence of GSNO, while proliferation of endothelial cells was promoted. In vitro platelet adhesion and arteriovenous shunt experiments demonstrated good antithrombotic properties of this material, with inhibited platelet activation and aggregation, and prevention of acute thrombosis. This study may provide a new method of improving cellular function and antithrombotic property of vascular grafts.

关 键 词：nitric oxide catalytic generation ELECTROSPINNING vascular graft BIOCOMPATIBILITY 

分 类 号：R318.08[医药卫生—生物医学工程]