肝郁与慢性非细菌性前列腺炎的关系  

Relationship between liver depression and chronic non-bacterial prostatitis

作　　者：孙洪福[1] 刘燕[2] 王卫东[1] 高兆旺[1] 张庆祥[2] 

机构地区：[1]山东中医药大学附属医院泌尿外科,山东济南250014 [2]山东中医药大学基础医学院,山东济南250011

出　　处：《中国医药导报》2015年第23期21-24,共4页China Medical Herald

基　　金：山东省中医药科技发展计划项目(2013-051)

摘　　要：目的建立慢性非细菌性前列腺炎(CNP)并肝郁大鼠模型的方法 ,探讨肝郁与CNP的关系。方法采用Wistar雄性大鼠50只,模型组为37只,前列腺蛋白制备组5只,空白组8只。其中模型组随机选取6只作为CNP组,6只作为肝郁并CNP组。先采用弗氏免疫佐剂法制备CNP模型,观察前列腺病理变化和肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)浓度变化,然后对模型组采用束缚法制备肝郁并CNP模型,观察前列腺病理变化和TNF-α、IL-1β浓度变化。结果采用弗氏免疫佐剂法能导致大鼠前列腺出现炎性改变和TNF-α、IL-1β浓度升高。CNP组和肝郁并CNP组大鼠前列腺组织TNF-α和IL-1β浓度均高于空白组,差异有统计学意义(P<0.05);肝郁并CNP组大鼠前列腺组织TNF-α和IL-1β浓度均高于CNP组,差异有统计学意义(P<0.05)。结论弗氏免疫佐剂法结合束缚法能制备肝郁并CNP模型,肝郁能加重CNP的炎性改变。Objective To establish method of chronic non-bacterial prostatitis（CNP） and liver depression rat model and probe into the relationship between liver depression and CNP. Methods 50 male Wistar rats were chosen for experiment, among which 37 rats were chosen for model group, 5 rats were chosen for preparation group and the other 8rats were chosen for blank group. 6 rats were randomly selected as CNP group and 6 rats as liver depression combined with CNP group in model group. First, the model of CNP were made by Freund′s adjuvant method to observe the pathological change of prostate and level change of TNF-α, IL-1β; then, the liver depression pattern of CNP was made by binding method to observe the pathological changes of prostate and level change of TNF-α, IL-1β. Results Freund′ s adjuvant method could lead to rat prostate inflammatory change and the level rising of TNF-α and IL-1β. TNF-α, IL-1β level of rat prostate in CNP group and liver depression combined with CNP group was higher than that in blank group respectively, with statistical difference（P〈0.05）. TNF-α, IL-1β level of rat prostate in liver depression combined with CNP group was higher than that in CNP group respectively, with statistical difference（P〈0.05）. Conclusion Freund′s adjuvant method combined with the binding method can prepare liver depression pattern of CNP, liver depression can aggravate inflammatory change of CNP.

关 键 词：慢性非细菌性前列腺炎 肝郁 病证结合模型 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]