NLRs模式识别受体在变应性鼻炎患者发病中的作用和意义  

The role of Nods like receptors in the patients with allergic rhinitis

作　　者：张沈华[1,2] 刘艳慧[3] 申聪香[1] 李冠雪[1] 杨柯柯 史欣[1] 文忠[1] 

机构地区：[1]南方医科大学珠江医院耳鼻咽喉-头颈外科,广州510282 [2]广东省第二人民医院耳鼻咽喉头颈外科,广州510317 [3]新疆医科大学第二附属医院耳鼻咽喉科 [4]河南省平顶山煤炭集团总医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第15期1323-1328,共6页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨核苷酸结合寡聚化结构域(NLRs)样模式识别受体家族Nod1、Nod2及Nalp3在变应性鼻炎(AR)患者发病中的作用和意义。方法:分别采用Real-Time RT-PCR、免疫组织化学及Western blot检测Nod1、Nod2、Nalp3蛋白在AR患者下鼻甲组织中的表达,以鼻中隔偏曲(NSD)患者下鼻甲黏膜作为对照,分别通过Western blot检测观察AR患者舌下脱敏治疗6个月后外周血单个核细胞中Nod1的改变,通过ELISA检测观察外周血IL-4、IL-6、IL-10、IFN-γ的变化。结果:3种Nods样受体在AR及NSD组鼻黏膜组织中均有表达,且主要表达于鼻黏膜上皮细胞、腺体上皮、炎性细胞(如浆细胞、嗜酸粒细胞)。但在AR组中Nod1mRNA及蛋白表达均低于NSD组(P<0.05),Nalp3mRNA及蛋白表达均高于NSD组(P<0.05);2组中Nod2mRNA、蛋白比较差异无统计学意义。脱敏治疗后6个月,Nod1表达改变与外周血IL-10的改变呈负相关(r=-0.88,P<0.05);Nod1表达改变与外周血IL-6的改变呈正相关(r=0.57,P>0.05)。结论:1Nod1、Nod2及Nalp3在2组组织中均有表达,但AR组Nod1表达低于NSD组,Nalp3高于NSD组,说明Nod1、Nalp3可能参与AR的发病。2舌下脱敏治疗6个月后,Nod1表达降低,其表达降低与外周血IL-10的改变呈负相关,提示Nod1可能通过调控IL-10变化参与舌下脱敏治疗机制。Objective：To explore role of Nods（nucleotide-binding oligomerization domain Nod Like receptors）kind of pattern recognition receptors（PRR）in patients with allergic rhinitis.Method：The mRNA and protein of Nod1,Nod2 of Nalp3were analyzed in the turbinate mucosa of patients with allergic rhinitis,nasal septum deviation（NSD）nasal mucosa of patients and nasal polyp mucosa with Real-Time RT-PCR,Western blot and immunohistochemistry respectively,and Nod1 expression changes was explored in PBMC with wad explored Western-blot and then the level of IL-4,IL-6,IL-10,IFN-γwere detected in serum of AR after desensitization treatment.Result：These Nods like receptors,mainly found in nasal mucosa epithelial cells,glandular epithelium and inflammatory cells（e.g.plasma cells,eosinophils）,were expressed in the nasal mucosa tissues.In AR group,Nod1（mRNA and protein）expression were lower than NSD group（P〈0.05）,Nalp3 expression were higher than（P〈0.05）,while,there was no significant difference of Nod2（mRNA and protein）between groups.After 6months desensitization therapy,the change of Nod1 in PBMC was negatively correlated with the change of IL-10 in the peripheral blood,r=-0.88,P〈0.05;while,change of Nod1 was positively correlated,with the change of IL-6,r=0.57,P〉0.05.Conclusion：Nod1,Nod2 and Nalp3expression were seen in the two groups,and the Nod1 expression in allergic rhinitis group was lower than other two groups,while,the Nalp3 was higher than other two groups.It showed Nod1,Nalp3 may be involved in the pathogenesis of allergic rhinitis.Expression of Nod1 in PBMC reduced after sublingual desensitization treatment.Besides,the change of Nod1 was negatively correlated with the change of IL-10 in PBMC.So,it seemed that Nod1 may regulate IL-10 changes and be involved in sublingual desensitization therapy.

关 键 词：Nods样模式识别受体 鼻炎 变应性 变应原舌下特异性免疫治疗 细胞因子 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]