滋阴疏肝法治疗大鼠肝纤维化的实验研究  

Experimental study on the method of nourishing Yin and dispersing stagnant liver qi in the treatment of liver fibrosis in rats

作　　者：白辰[1] 车念聪[1] 刘文兰[1] 司远[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《北京中医药》2015年第7期577-580,F0003,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金课题项目(81273668)

摘　　要：目的观察滋阴疏肝法对大鼠肝纤维化的治疗作用。方法 28只SD雄性大鼠,随机分为正常组、模型组、阳性药物组、中药组,每组7只。除正常组外,其余3组均建立四氯化碳诱导的肝纤维化模型。造模结束后,阳性药物组予秋水仙碱0.25 mg/kg灌胃,中药组予一贯煎浓缩液1 m L/100 g灌胃,正常组和模型组均以等量生理盐水灌胃,每日1次。每周记录大鼠体重,灌胃4周后检测大鼠体重、肝重、脾重、肝组织羟脯氨酸含量,计算肝重指数、脾重指数,通过病理染色评价肝纤维化程度。结果与正常组比较,模型组肝脏炎症和纤维化程度增加(P<0.01),体重降低,肝、脾重指数升高(P<0.01),肝脏羟脯氨酸含量升高(P<0.05,P<0.01)。与模型组比较,阳性药物组与中药组肝脏炎症和纤维化程度减轻(P<0.01),脾重指数及肝脏羟脯氨酸含量降低(P<0.05,P<0.01);肝重指数降低,体重增加,但差异无统计学意义(P>0.05)。结论滋阴疏肝法可减轻四氯化碳诱导的大鼠肝纤维化程度,缓解肝脾肿大。Objective To investigate the therapeutic effect of method of nourishing yin and dispersing stagnant liver qi on hepatic fibrosis in rats. Methods 28 SD male rats were randomized into the normal group,model group,positive drug group and Chinese herbal medicine group of 7 in each group. Except the normal group,carbon tetrachloride（ CCl4）-induced model of hepatic fibrosis was established in other three groups. After modeling,the positive drug group was given colchicines of 0. 25 mg / kg with gavage method,the Chinese herbal medicine group was given Yiguanjian concentrated liquid of 1 m L /100 g with gavage method,while the normal group and the model group were given equal amount of physiological saline once a day. The body weight of rats was monitored every week. After 4 weeks,the weight of body,liver and spleen,and the contents of hydroxyproline in liver tissue of rats were detected,the liver weight index and spleen weight index calculated,and the condition of liver fibrosis evaluated by pathological staining. Results Compared with the normal group,the condition of hepatitis and hepatic fibrosis became aggravated（ P〈0. 01）,the body weight decreased,the liver index and spleen index raised（ P〈0. 01）,liver hydroxyproline increased（ P〈0. 05,P〈0. 01） in the model group. Compared with the model group,the condition of hepatitis and hepatic fibrosis became alleviated,the spleen weight index and liver hydroxyproline were decreased（ P〈0. 01） in the positive drug group and Chinese herbal medicine group; the liver weight index dropped and the body weight increased,but the difference had no statistical significance（ P〉0. 05）. Conclusion The method of nourishing Yin and dispersing stagnant liver qi can reduce the degree of hepatic fibrosis in rats induced by carbon tetrachloride and alleviate the liver and spleen enlargement.

关 键 词：滋阴疏肝法 一贯煎 肝重指数 脾重指数 肝脏炎症 肝纤维化 大鼠 

分 类 号：R259[医药卫生—中西医结合]