基于groEL基因序列鉴定丹毒丝菌属菌种  

Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences

作　　者：杨振龙[1] 吾鲁木汗.那孜尔别克 恩特马克.布拉提白 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]伊犁师范学院生物与地理科学学院,新疆伊宁835000

出　　处：《吉首大学学报（自然科学版）》2015年第4期54-58,共5页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(31072142;31360613);湖南省科技厅科技计划资助项目(2009FJ3215)

摘　　要：对4株红斑丹毒丝菌的16SrRNA基因和groEL基因进行测序及系统发育分析,探讨groEL基因序列分析法在丹毒丝菌属菌种分类鉴定中的应用.测序结果表明,4株红斑丹毒丝菌groEL基因长度为1 614bp,编码由537个氨基酸残基组成热休克蛋白HPS60,与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株groEL基因的同源性分别为99%和86%,而16SrRNA基因长度为1 351bp,与红斑丹毒丝菌ATCC19414株和扁桃体丹毒丝菌ATCC43339株的同源性均为99%.系统发育分析结果表明,groEL基因比经典的16SrRNA基因序列分析分辨率高,可适用于红斑丹毒丝菌和扁桃体丹毒丝菌的分类鉴定.To investigate the effect of 16S rRNA gene and groEL gene used in the classification and identi-fication of species of the genus Erysipelothrix ,the 16S rRNA and groEL genes of four E .rhusiop athiae strains were amplified by PCR and sequenced and their phylogenetic threes was constructed .The open reading frame of groEL genes of four tested strains were 1 614 bp in length ,and sequence similarities of the groEL gene between the four tested strains and the previously reported E .rhusiopathiae type strain ATCC19414 and E .tonsillarum type strain ATCC43339 were 99% and 86% ,respectively .While the 16S rRNA genes of the four tested strains was 1 351 bp in length ,and sequence similarity of the 16S rRNA gene between the four tested strains and E .rhusiopathiae type strain ATCC19414 and E .tonsillarum type strain ATCC43339 was 99% .Phylogenetic analysis indicated that the E .rhusiopathiae and E .tonsillarum were differentiated well by groEL gene sequence analysis .This study demonstrated that the groEL gene is an alternative phylogenetic marker for the classification of species of the genus Erysipelothrix .

关 键 词：红斑丹毒丝菌 扁桃体丹毒丝菌 16S RRNA基因 groEL基因 分类鉴定 

分 类 号：Q939.1[生物学—微生物学]