两株不同致病力H9N2亚型禽流感病毒全基因组序列分析  

Complete Genome Sequence Analysis of Two H9N2 Subtype Avian Influenza Virus strains Bearing Different Virulences

作　　者：刘琳琳[1] 张毅[2] 郭学金[1] 范丹丹[1] 朱梅胜 王守春[1] 徐守振[1] 王建琳[1] 毕玉海[3] 尹燕博[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]中国科学院微生物研究所,北京100101

出　　处：《动物医学进展》2015年第9期37-42,共6页Progress In Veterinary Medicine

基　　金：山东省现代农业产业技术体系家禽创新团队建设项目(SDAIT-13-011-03);国家自然科学基金项目(31272535)

摘　　要：为了找到H9N2亚型禽流感病毒毒株致病力增强的分子线索,对致病力存在明显差异的2株H9N2亚型禽流感病毒进行了全基因组序列测定和分析。遗传进化分析表明,2株H9N2亚型禽流感病毒发生了基因重配,内部基因来源具有多样性。氨基酸序列分析表明,强致病力毒株A/chicken/Shandong/818/2010和A/chicken/Shandong/196/2011在HA受体结合位点、NA唾液酸结合位点和潜在糖基化位点处存在显著差异,SD/818在PA蛋白55位氨基酸和336位氨基酸等重要功能位点处出现了与致病力增强有关的突变,这种致病力增强的分子机制需要进一步探究。In order to find the molecular clues of higher pathogenicity of H9N2 AIVs, we determined the full-genome sequences of two H9N2 strains bearing different pathogenicities to chickens. Phylogenetic anal- ysis showed that the reassortment may occur in the internal genes of the two viruses. Amino acid sequence analysis showed one of the receptor binding sites of HA was different between the two viruses. A/chicken/ Shandong/818/2010 beard one more potential glycosylation site of NA,and showed difference in one of the sialic acid binding sites. The mutations of 55aa and 336aa in PA,which are related to higher replication abil- ity and pathogenicity,were identified in the virulent strain of A/chicken/Shandong/818/2010. The molecu- lar mechanism behind need to be further verified and researched.

关 键 词：H9N2亚型禽流感病毒 致病力 全基因 序列分析 遗传进化 

分 类 号：S852.659.6[农业科学—基础兽医学]