丙型病毒性肝炎抗病毒治疗无应答和复发患者白细胞介素-28B基因多态性分析  

Gene polymorphisms of interleukin-28B in no-response and relapse patients with chronic hepatitis C treated by antiviral therapy

作　　者：宁会彬 何佳[1,2] 刘俊平[1,2] 彭真[1,2] 毛重山[1,2] 康谊[1,2] 尚佳[1,2] 

机构地区：[1]郑州大学人民医院 [2]河南省人民医院感染性疾病科,郑州450003

出　　处：《中华实用诊断与治疗杂志》2015年第8期749-751,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家重点专科建设项目(2013);河南省科技攻关计划省部共建项目(201401015);河南省科技攻关普通项目(201303149);河南省科技攻关普通项目(201003107)

摘　　要：目的探讨丙型病毒性肝炎抗病毒治疗无应答及复发患者白细胞介素-28B基因的多态性。方法丙型病毒性肝炎患者73例,均采用聚乙二醇干扰素联合利巴韦林标准方案治疗,其中抗病毒治疗复发者26例为复发组,无应答者22例为无应答组,获得持续病毒学应答(sustained virological response,SVR)者25例为SVR组。采用基因测序法检测3组白细胞介素-28B基因多态性位点rs12979860和rs8099917,比较3组各位点基因型分布情况。结果无应答组和复发组白细胞介素-28B基因rs12979860CT基因型比例(77.3%,57.7%)和rs8099917TG基因型比例(77.3%,57.7%)明显高于SVR组(8.0%,8.0%)(P<0.05),rs12979860CC基因型比例(18.2%,42.3%)和rs8099917TT基因型比例(18.2%,42.3%)明显低于SVR组(92.0%,92.0%)(P<0.05),无应答组与复发组比较差异均无统计学意义(P>0.05);73例中rs12979860CT杂合基因及rs8099917TG杂合基因者均为34例,其中复发者和无应答者共32例(94.15%),明显高于SVR患者2例(5.85%)(P<0.05)。结论丙型病毒性肝炎患者抗病毒治疗无应答及复发与其白细胞介素-28B基因多态性密切相关。Objective To discuss the gene polymorphisms of interleukin-28B （IL-28B） in no-response and relapse patients with chronic hepatitis C treated by antiviral therapy. Methods Seventy-three patients were treated with standard interferon scheme, including 26 patients with relapse （relapse group）, 22 patients with no response （no-response group） and 25 patients with sustained virological response （SVR group）. Gene sequencing method was used to detect the distributions of IL-28B polymorphism rs12979860 and rs8099917, which were compared among three groups. Results The rates of IL-28B rs12979860 CT （77.3∽/00, 57.7%） and rs8099917 TG （77.3%, 57.7%） were higher in no-response group and relapse group than those in SVR group （8.0%, 8.0%） （P〈0.05）. The rates of rs12979860 CC （18. 2∽if0, 42.3%//00） and rs8099917 TT （18.2%, 42.3%） were significantly lower in no-response group and relapse group than those in SVR group （92.0%, 92.0%） （P〈0.05）. There were no significant differences between non-response group and relapse group （P〈0.05）. Relapse and no-response occurred in 32 patients （94. 15%） out of 34 CT hybrid genes, significantly more than SVR patients （n = 2, 5. 85 %） （P〈 0.05）. Conclusion No-response and relapse are closely correlated with IL-28B gene polymorphism in chronic hepatitis C patients treated by antiviral therapy.

关 键 词：慢性丙型病毒性肝炎 疗效 白细胞介素-28B 基因多态性 

分 类 号：R512.63[医药卫生—内科学]