载VEGFR-3抗体淋巴管靶向微泡的体外寻靶试验研究  

Targeting study of VEGFR-3 antibody loaded microbubble in vitro

作　　者：杨阳[1] 朱梅[1] 刘政[2] 章晓锋 严飞[3] 赵溪岩[4] 

机构地区：[1]昆明医科大学第一附属医院超声科/云南省超声影像研究中心,云南昆明650032 [2]第三军医大学新桥医院超声科,重庆400037 [3]中国科学院深圳先进技术院,广东深圳518055 [4]海南医学院机能实验室,海南海口571199

出　　处：《中国生化药物杂志》2015年第3期10-12,17,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(81160178)

摘　　要：目的制备载VEGFR-3抗体的淋巴管靶向微泡,观察体外寻靶效果。方法采用生物素-亲和素桥接法制备载VEGFR-3抗体靶向微泡,进行体外寻靶实验,采用分组对照实验以直接、间接法观察寻靶效果。直接法使用图像分析软件IPP进行离线分析及采用SPSS软件包进行统计学分析;间接法采用分组孵育、离心法直观观察靶向结合效果。结果图像软件IPP离线分析结果显示靶向组微泡与淋巴管内皮细胞结合数量明显多于普通组;高倍显微镜下(×100倍)靶向组平均每个视野有(151±20)个靶向微泡粘附,普通组平均每个视野有(12±4)个普通微泡粘附,靶向组与普通组微泡结合数量差异有统计学意义(t=19.19,P<0.01)。间接观察法结果显示:与普通组相比,靶向组中靶向微泡与细胞能逐步牢固的结合。结论制备的载VEGFR-3抗体淋巴管靶向微泡体外寻靶效果明显优于普通微泡。Objective To prepare targeted microbubbles which load anti-VEGFR-3 and observe the targeting effect in vitro.Methods Targeted microbubbles loaded VEGFR-3 antibody were prepared and the targeting effect in vitro were tested by direct and indirect methods.Direct method took off-line analysis by image analysis software IPP and statistical analysed with SPSS software package.Indirect method took direct observation the target binding effect after incubation and centrifugal sedimentation.Results Image software IPP results showed that the quantity of microbubbles in targeted-group was obviously more than ordinary group.The average microbubbles per field in targeted-group was （151 ±20）.The average microbubbles per field in ordinary group was （ 12 ±4 ） microbubbles, the numbers of combined microbubbles between targeted-group and ordinary-group had significant differences （ t=19.19,P〈0.01）.The indirect method results showed that, compared with ordinary group, the microbubbles in targeted-group could combined with cell gradually.Conclusion Targeted microbubbles load anti-VEGFR-3 have a better contrast effect than common microbubbles.

关 键 词：VEGFR-3抗体 微泡 寻靶 体外实验 

分 类 号：R318[医药卫生—生物医学工程]