当归多糖与川芎嗪不同配比对大鼠脑缺血再灌注损伤神经可塑性的影响  

Neuroplasticity effects of compatibility of angelica sinensis polysaccharide and tetramethylpyrazine on cerebral ischemia/reperfusion injury in rats

作　　者：刘洛同[1] 明扬[1] 陈礼刚 周杰[1] 彭里磊[1] 孙霞[2] 

机构地区：[1]泸州医学院附属医院神经外科,四川泸州646000 [2]山东大学分子药理学系,山东济南250100

出　　处：《中国生化药物杂志》2015年第3期36-38,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：山东省医药卫生科技发展计划项目(2009QW003)

摘　　要：目的研究低分子量当归多糖-川芎嗪(LMW-ASP)不同配比对大鼠脑缺血再灌注损伤后神经可塑性的影响。方法将42只实验大鼠随机分为对照组(N)、假手术组(S)、模型组(M)、50∶10组(mg/kg∶mg/kg)(E1)、25∶10组(E2)、50∶20组(E3)、25∶20组(E4)。N组不进行任何手术处理,S组除不插线栓外,余同模型组,M、E1、E2、E3、E4组大鼠建立MCAO再灌注模型,其中N、S、M组不给药治疗,腹腔注射相同剂量的生理盐水,E1-E4分别腹腔注射相应比例的低分子量当归多糖和川芎嗪,于再灌注即刻开始注药,1次/日,共7次。分别在再灌注后4 h、24 h、3 d、7 d进行大鼠神经功能缺损评分,并于第7 d多聚甲醛固定后断头取脑进行免疫组织化学方法检测脑梗死周围皮质MAP-2、SYP的表达。结果免疫组织化学方法检测脑梗死灶周围皮质MAP-2、SYP表达显示,在第7d,建立脑缺血再灌注损伤模型的各组MAP-2、SYP的免疫活性较N组、S组强,药物干预组MAP-2、SYP的免疫活性高于M组,且差异均具有统计学意义(P<0.05);低分子量当归多糖-川芎嗪不同配比组间MAP-2的免疫活性差异有统计学意义,其活性分别为E3>E1>E4>E2;低分子量当归多糖-川芎嗪不同配比组间SYP的免疫活性差异均有统计学意义,其活性分别为E3>E1>E4>E2。其比例为50 mg/kg∶20 mg/kg时最适宜。结论大鼠脑缺血再灌注损伤后神经功能部分可自行恢复,低分子量当归多糖-川芎嗪配伍干预后可促进大鼠脑缺血再灌注损伤脑梗死灶周围皮质MAP-2、SYP的表达,恢复效果更显著。Objective To construct a middle cerebral artery occlusion （ MCAO ）/reperfusion rat model and study the effect of the different compatibility ratios of LMW-ASP and tetramethylpyrazine on the effects of neuroplasticity, and explore the best compatibility ratio between LMW-ASP and tetramethylpyrazine.Methods In the study, 42 experimental rats were divided into seven groups, they were normal group（N）, sham operation group （S）, model group（M）,50：10 group（E1）,25：10 group（E2）,50：20 group（E3） and 25：20 group（E4）.N group would not be carried out any operation, while S group was performed in the same way with M group but without inserting the intraluminal suture.MCAO reperfusion models were established in rats of the M,E1,E2,E3,E4 groups.The corresponding compatibility ratios of LMW-ASP and tetramethylpyrazine were injected to the E1-E4 groups by intraperitoneal injection in reperfusion instantly,once a day,a total of seven times.Neurological severity scores in rats were done in 4 h, 24 h,3 d,7 d after reperfusion.In 7 d, The rats brains were removed after fixed using paraformaldehyde and then the immunohistochemistry method was used to detect the expression of MAP-2 and SYP in the cortex around cerebral infarction.Results In 7 d, using the immunohistochemical methods detected the expression of MAP-2 and SYP in the cortex around cerebral infarction.The immune activity of MAP-2 and SYP in groups of cerebral ischemia/reperfusion injury model was stronger than the N group, S group and the difference was significant（P 〈0.05）, and the immune activity of MAP-2 and SYP in the cortex around cerebral infarction in drug intervention groups were stronger than the M group and they had a significant difference （ P 〈0.05 ）; the immune activity of MAP-2 between the different compatibility ratios groups of LMW-ASP and tetramethylpyrazine was statistical difference（P〈0.05）, and the activity was E3〉E1〉E4〉E2;the immune activity of SYP between the different compatibility ratios g

关 键 词：当归多糖 川芎嗪 大脑缺血再灌注模型 

分 类 号：R285.5[医药卫生—中药学]