当归补血汤对吗啡戒断小鼠红细胞免疫功能的影响研究  

Effect of Danggui Buxue Decoction on red cell immune function in mice with morphine abstinence syndrome

作　　者：顾鸿[1] 田维毅[2] 梅颖[3] 

机构地区：[1]贵阳中医学院实验针灸教研室,贵州贵阳550002 [2]贵阳中医学院免疫教研室,贵州贵阳550002 [3]贵阳中医学院中药教研室,贵州贵阳550002

出　　处：《中国生化药物杂志》2015年第3期51-53,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：贵州省科技厅攻关项目([2007]1039)

摘　　要：目的研究当归补血汤对吗啡戒断小鼠红细胞免疫功能的影响。方法将50只实验小鼠随机分为5组:正常组、阳性对照组、药物高、中、低剂量组。后肢注射吗啡建立小鼠吗啡成瘾模型。红细胞计数仪测量5组小鼠的红细胞膜微粘度(erythrocyte membrane micro-viscosity,EM-MV)、红细胞变形指数(erythrocyte deformation index,EDI);采用郭氏法测定红细胞免疫复合物花环率(red blood cell-immune complex rosette,RBC-ICR)、红细胞C3b受体花环率(red blood cell-C3b receptor rosette,RBC-C3bRR);并观察5组红细胞免疫黏附促进因子(red blood cell regulator factor enhance rate,RFER)和红细胞免疫黏附抑制因子(red blood cell immune adherence inhibitor factor,RFIR)变化水平。结果阳性对照组EM-MV均显著高于正常组、药物中剂量组和药物低剂量组(P<0.05),阳性对照组EDI显著低于正常组、药物中剂量组和药物低剂量组(P<0.05)。药物中剂量组和低剂量组较阳性对照组明显RBC-ICR降低(P<0.05),RBC-C3bRR显著高于阳性对照组(P<0.05);药物中剂量组和低剂量组RFER水平显著高于阳性对照组,RFIR显著降低RFER(P<0.05)。结论低剂量组和高剂量组当归补血汤提高吗啡戒断小鼠的红细胞免疫功能,改善戒断的症状,促进机体的快速康复,具有良好的临床应用价值。Objective To study the effect of Danggui Buxue Decoction on red cell immune function in mice with morphine abstinence syndrome. Methods 50 mice were randomly divided into normal group,positive control group, high, medium, low dose group,Hindlimb injection of morphine in morphine dependent mice model was established.Erythrocyte membrane were measured in five groups of mice micro viscosity, erythrocyte deformation index; Determination of red cell immune complex rosette rate using the method （CIC）, red cell C3b receptor rosette rate;and to observe the five group of regulator factor enhance rate （RFER） and immune adherence inhibitor factor （RFIR） levels.Results Erythrocyte membrane microviscosity in model group were significantly higher than those in normal group, medium group and low group （P〈0.05）,red cell deformability index was lower than that of normal group, drug medium group and drug group low （P〈0.05）.Drug high group and medium group,immune complex rosette rate lower than in model group（P〈0.05）, C3b receptor rosette rate was significantly higher than that in the model group （P〈0.05）.Conclusion Low dose group and high dose group Danggui Buxue Decoction raises the red cell immune function in morphine withdrawal mice, improves the withdrawal symptoms, rapids rehabilitation of the body, has good clinical value.

关 键 词：当归补血汤 吗啡戒断 红细胞免疫 

分 类 号：R245.81[医药卫生—针灸推拿学]