副猪嗜血杆菌LN/111013株sod A基因序列分析及其毒力强度初步判断  

Sod A gene sequence analysis of Haemophilus parasuis named LN/111013 and pathogenic preliminary judgment

作　　者：程迪[1] 迟阳 艾春华 董筱萍[1] 高慎阳[1] 刘丽颖 周铁忠[1] 王佳丽[1] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]艾迪康医学院检验中心,辽宁沈阳110000 [3]锦州市古塔区动物卫生监督所,辽宁锦州121001 [4]营口市动物疫病预防控制中心,辽宁营口115000

出　　处：《中国兽医学报》2015年第8期1223-1227,共5页Chinese Journal of Veterinary Science

基　　金：辽宁省十二五科技攻关重点资助项目(2011214001);辽宁省百千万人才工程资助项目(2014921012);辽宁省教育厅大学生创新创业训练计划资助项目(201310160037)

摘　　要：根据已经发表的副猪嗜血杆菌（HPS）sod A基因序列设计引物,对辽宁省HPS LN/111013株sod A基因进行特异性扩增、克隆及测序,获得长度为621bp的sod A基因完整序列。通过与已知代表毒株的sod A基因核苷酸及其编码的氨基酸进行同源性比对和系统进化树分析,结果显示核苷酸的同源性为97.3%～100.0%,氨基酸的同源性为95.2%～100.0%,表明该毒株与近年来国内外流行毒株的亲缘关系较近。通过与国内标准HPS强毒株（SH0165）sod A基因序列进行比对,发现该辽宁株核苷酸7个主要位点中在206,516,556处一致,而在48,147,450,597这4个位点不一致;氨基酸4个关键位点中16,49位点符合低致病力毒株特点,69,186位点符合高致病力毒株特点。而该毒株的临床发病情况和致病性试验表明其毒力较强,说明仅凭一个sod A毒力基因来判断该菌株的致病性还不够全面,或该辽宁株可能来源于高低菌株的变异或重组。The primer was designed according to the published HPS sodA gene sequences,the sod A gene of HPS strain isolated from Liaoning province were amplified by PCR,cloned,sequenced and obtained the 621 bp product containing the complete sequence of sod A gene.The nucleotide homology of sod A gene were up to 97.3%-100.0% and the amino acid homology were up to95.2%-100.0% with other known representatives of HPS strains,indicating that the strain is similar to the prevalent HPS strains in domestic and international.The site of 206,516 and 556of the sod A gene were the same,the site of 48,147,450 and 597were diffrent compared with virulent HPS standard strain（SH0165）;in the four key sites of the amino acid,the 16 th and 49 th site were in accord with highly pathogenic sites,the 69 th and 186 th site were in accord with low pathogenic sites.However,the clinical infection situation and pathogenicity test of the HPS LN/111013 stain showed its virulent,indicating that it is not comprehensive to judge the pathogenic of the stian only on the basis of the sod A gene,or the stain isolat from Liaoning province may derived from mutation or recombination of gene.

关 键 词：副猪嗜血杆菌 SOD A基因 变异分析 毒力 

分 类 号：S852.61[农业科学—基础兽医学]