姜黄等4种具有抗真菌活性中药的最佳提取工艺  

Optimal extraction process of four traditional Chinese medicine with antifungal activity such as Curcuma longaL.

作　　者：张悦天 李曼[1] 向发 殷中琼[1] 贾仁勇[1,2] 余婷婷[1] 陈静[1] 朱维凤[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]预防兽医研究所,四川雅安625014

出　　处：《中国兽医学报》2015年第8期1317-1323,共7页Chinese Journal of Veterinary Science

基　　金："十二五"农村领域国家科技计划资助项目(2011BAD34B03);水禽疫病控制四川省青年科技创新研究团队资助项目(2013TD0015)

摘　　要：以浸膏得率、最低抑菌浓度(MIC)值为指标,采用正交设计试验,综合考虑提取方法、溶剂、料液比、提取次数、提取时间、提取温度等5个影响因素,对姜黄等4种具有抗真菌活性中药有效物质提取工艺进行优选。结果优选出的最佳提取工艺分别为:姜黄粗粉(20目)80%乙醇溶液(6倍量),加热回流提取3次,每次2h;射干粗粉(20目)70%乙醇溶液(10倍量),加热回流提取3次,每次2h;栀子粗粉(20目)80%甲醇溶液(6倍量)浸泡0.5h后超声波提取2h;五倍子粗粉(20目)48%乙醇溶液(23倍量),59℃条件下超声提取29min。最佳提取工艺下,姜黄、射干、栀子、五倍子4种提取物浸膏对4株真菌的MIC总值和分别达到31.25,25.00,18.75,18.75g/L,抑菌效果明显且提取工艺稳定可行,值得借鉴。To optimize the extracting process of active substance in four traditional Chinese medicines.With extraction rate and MIC as the index,the following five factors were considered：extraction method,concentration dosage of extraction solvent,extracting times,extracting time,and extracting temperature,the extracting process of active substance in four traditional Chinese medicine were optimized by the orthogonal.The optimization conditions of the extracting process were that the coarse powder of Curcuma longaL.（20sieve）,80%ethanol（6times）as solvent,heating reflux extraction 3times,each time for 2h;the coarse powder of Belamcanda chinensis（L）DC.（20sieve）,70% ethanol（10times）as solvent,heating reflux extraction 3times,each time for 2h;the coarse powder of Gardenia jasminoides Ellis（20sieve）,80% methanol solution（6times）as solvent,soaking half an hour and then ultrasonic extraction for 2h;the coarse powder of Gallnut（20sieve）,48% ethanol（23times）as solvent,under the condition of 59℃ ultrasonic extraction for29 min.Under the optimal extraction process,the MIC of four kinds of extracts against four strains of fungi are 31.25,25.00,18.75,18.75g/L,respectively.The result showed that bacteriostatic effect of the four extracts is obvious and the extraction process is stable,feasible.

关 键 词：中药 有效物质 提取工艺 正交设计试验 

分 类 号：S859.3[农业科学—临床兽医学]