当归补血汤对异丙肾上腺素诱导大鼠心肌纤维化的影响  

Influence of Danggui Buxue Decoction on Myocardial Fibrosis of Isoproterenol Induced Rats

作　　者：曾宇[1] 张三印[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《云南中医中药杂志》2015年第7期17-21,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金项目(81001497);成都中医药大学校基金项目(030018060)

摘　　要：目的探讨当归补血汤对异丙肾上腺素诱导大鼠心肌纤维化的影响。方法 50只SD大鼠随机分为空白对照组、模型对照组、当归补血汤大剂量组、当归补血汤小剂量组、阳性对照组。除空白对照组,其它四组大鼠给予背部皮下注射异丙肾上腺素5 mgkg-1d-1,连续10天。阳性对照组给予卡托普利50 mg/kg-1灌胃,当归补血汤大和小剂量组给予当归补血汤各7.56 g/kg-1和3.78 g/kg-1灌胃,空白对照组和模型对照组给予等体积的生理盐水灌胃,每天一次。连续给药28d后,给予血流动力学检测,计算心脏指数,病理检测,Hpy含量检测。结果与空白对照组比较,模型对照组的心脏指数升高(P<0.05),左心功能降低,Hpy含量增加(P<0.01)。与模型对照组比较,当归补血汤大剂量、小剂量组大鼠心肌纤维化病变程度降低,心脏组织的Hpy含量降低,左心功能有改善。结论当归补血汤能抑制心肌间质细胞产生胶原蛋白,减轻心肌纤维化,改善左心功能。Objective： To study the influence of Danggui Buxue Decoction （DBD） on myocardial fibrosis of iso- proterenol -induced rats. Methods： 50 SD rats were randomly divided into a normal control group, a model group, a DBD high -dose group, a DBD low -dose group and a positive control group. Except the normal control group, the rats of other four groups were given subcutaneous injection of isoproterenol 5 mg/kg-1 d-1 for 10 days. The positive were given intragastric infusion of model group were given intragastri 50 mg/kg-1. The DBD high- dose and low- dose groups DBD 7.56 g/kg-~ and 3.78 g/kg-1 respectively. The normal control group and the c infusion of equivoluminal？ normal saline, once a day. After 28 - day successive administration, hemodynamics detection, cardiac index calculation, pathological examination and Hpy content detec- tion were made. Results： When compared with the normal control group, the cardiac index of the model group in- creased （ P 〈 0. 05 ）, their left ventricular function decreased and Hpy content increased （ P 〈 0. 01 ）. When compared with the model group, the DBD high - dose and low - dose groups reduced the severity of myocardial fibrosis lesion of the rats and their Hpy content of heart tissue decreased and their left ventricular function improved. Conclusion ： DBD can inhibit myocardial interstitial cells to produce collagen, reduce myocardial fibrosis and improve left ventricular function.

关 键 词：心肌纤维化 当归补血汤 羟脯氨酸 

分 类 号：R541[医药卫生—心血管疾病]