B[a]P和DDT亚急性联合暴露对小鼠肝功能酶ALT、AST和γ-GT的影响及作用形式  

Effects of benzo[a]pyrene and DDT subacute co-exposure on ALT, AST and γ-GT in mice

作　　者：赵苒[1] 刘敏[1] 王娟[1] 郑芳[1] 郭东北[1] 范春[1] 

机构地区：[1]厦门大学公共卫生学院,福建厦门361102

出　　处：《癌变．畸变．突变》2015年第4期272-277,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：福建省自然科学基金项目(2013J01354);厦门市科技计划项目(3502Z20123003);中央高校基本科研业务费专项资金(2011121006)

摘　　要：目的:探讨不同剂量的苯并[a]芘(B[a]P)和滴滴涕(DDT)联合暴露对小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)和γ-谷胺酰转移酶(γ-GT)的影响及作用形式。方法:成年雄性昆明种小鼠50只,随机分为10组即空白对照组(正常饲养)、溶剂对照组(食用油和二甲基亚砜处理)、低和高浓度B[a]P染毒组[1.0和10 mg/(kg·d)]、低和高浓度DDT染毒组[0.6和6 mg/(kg·d)]、低浓度DDT+低浓度B[a]P染毒组、低浓度DDT+高浓度B[a]P染毒组、高浓度DDT+低浓度B[a]P染毒组、高浓度DDT+高浓度B[a]P染毒组。染毒组各受试物经腹腔注射染毒,每日注射1次,连续31 d。末次染毒24 h后通过眼球摘除取血,自动生化仪检测血清中ALT、AST、γ-GT的活性;并制作肝脏HE切片,观察肝细胞形态。采用两因素三水平析因设计的方差分析对数据进行统计分析。结果:与对照组比较,B[a]P和DDT单独染毒时,均能诱导ALT和AST活性升高(F=41.308,P=0.000;F=20.083,P=0.000),随各自染毒剂量的增加,ALT和AST活性升高,但二者联合暴露对ALT和AST活性均不存在交互作用(分别为P=0.258,P=0.264)。B[a]P和DDT单独染毒对γ-GT活性均未产生明显影响,联合暴露也不存在交互作用(P=0.816)。HE染色观察到肝细胞膜界限模糊,发生水样变性,肝细胞中出现小空泡,呈蜂窝状,随染毒剂量增加,肝细胞质水样变性加剧,部分肝细胞质溶解,并且细胞核肿大,形状不规则。结论:在本实验条件下,B[a]P和DDT单独染毒均能诱导小鼠血清ALT和AST活性升高,不同剂量的B[a]P和DDT联合暴露对小鼠血清ALT、AST活性的作用形式主要表现为单独作用,而非交互作用。B[a]P和DDT的单独和联合暴露均未观察到对γ-GT的活性产生明显影响。OBJECTIVE： To explore the effects of benzo[a] pyrene and DDT exposure alone or in combination on ALT, AST and γ-GT in mice. METHODS： Fifty healthy male Kunming mice were divided into 10 groups at random： blank control group （normal feed）; solvent control group（equal volume of oil）; 1, 10 mg/（kg· d） B[a]P groups; 0.6, 6 mg/（kg· d） DDT groups; 1 mg/（kg· d） B[a]P＋0.6 mg/（kg·d） DDT group; 1 mg/（kg· d） B[a]P＋6 mg/（kg · d） DDT group; 10 mg/（kg · d） B[a]P＋0.6 mg/（kg· d） DOT group and 10 mg/（kg · d） B[a]P＋6 mg/（kg· d） DDT group. Exposur6 groups were treated with oil contained B[a]P and DDT once a day. After 31 days of intraperitoneal injection, eyeballs were extracted and ALT, AST and γ-GT were examined in blood. HE slices were made to confirm the liver damage. Using two factors and three levels factorial ANOVA design for data analysis. RESULTS ： The levels of ALT and AST in all B[a]P or DDT alone exposure groups were significantly higher than that of the control groups （F=41.308, P=0.000; F=20.083, P=0.000）, showing dose-effect relationships in both. But no interaction was observed in co-exposure groups（P=0.258, P=0.264）. No significant changes in γ-GT were observed in all B[a]P or DDT alone exposure groups. Neither was there any interactive effect in co-exposure groups（P=0.816）. This was in accordance with the observation in HE slice： hydropic degeneration, endolysis and nuclear enlargement in liver cell. CONCLUSION： Under this experimental condition, increasing levels of ALT and AST could be detected in B[a]P or DDT separate exposure groups. But neither could affect γ-GT level. No interactive effects of ALT, AST and γ-GT could be induced in co-exposure groups.

关 键 词：苯并[A]芘 滴滴涕 联合暴露 谷丙转氨酶 谷草转氨酶 γ-谷胺酰转移酶 交互作用 

分 类 号：R994.6[医药卫生—毒理学]