胃炎Ⅰ号对胃癌前病变大鼠胃黏膜NF-κBp65、IKKβ mRNA、VEGF表达的影响  

Effects of Weiyanyihao on expression of NF-κBp65, IKKβ mRNA, VEGF in rat gastric mucosa with precancerous lesions of gastric cancer

作　　者：李海文[1] 潘华峰[1] 曾进浩[1] 赵自明 史亚飞[1] 袁玉梅[1] 林钟宇 赵金媛[1] 

机构地区：[1]广州中医药大学,广州510006 [2]广东省中医药工程技术研究院,广州510095

出　　处：《中华中医药杂志》2015年第8期2945-2948,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81273739);广东省联合培养研究生示范基地人才培养项目(No.2013JDXM20)~~

摘　　要：目的:探讨健脾化瘀解毒方胃炎Ⅰ号对胃癌前病变(PLGC)大鼠胃黏膜NF-κBp65、IKKβm RNA、VEGF表达的影响。方法:将SD大鼠随机分为正常组、模型组、维酶素组、胃炎Ⅰ号组。除正常组外,其他组采用MNNG饮水液自由饮用(200μg/m L)、饥饱失常、小承气汤(2m L/只,生药1g/m L)灌胃法复制PLGC大鼠模型,使用胃炎Ⅰ号灌胃10周后,检测胃黏膜上皮细胞NF-κBp65、IKKβm RNA、VEGF的表达。结果:模型组胃黏膜NF-κBp65、IKKβm RNA、VEGF的表达较正常组显著升高(P<0.01);胃炎Ⅰ号可降低NF-κBp65、IKKβm RNA、VEGF的表达评分(P<0.01)。结论:胃炎Ⅰ号能抑制NF-κB及VEGF信号通路的异常激活,下调NF-κBp65、IKKβm RNA、VEGF的表达,能一定程度抑制胃黏膜慢性炎性反应及血管新生,起到防治胃癌前病变的作用。Objective： To observe the effects of Weiyanyihao on the expression of NF-κBp65, IKKβ mRNA, VEGF in rat gastric mucosa with precancerous lesions of gastric cancer （PLGC）. Methods： The SD rats were randomized into four groups, including normal, model, Vatacoenayme and Weiyanyihao. To prepare the rat model of PLGC by giving MNNG （200μg/mL） and basal diet, Xiaochengqi Decoction （2mL per） by gavage and abnormal of starvation and full. Results： Compared with the normal, the NF-κBp65, IKKβ mRNA, VEGF in the model group were increased （P〈0.01）; Compared with the model group, the NF- κBp65, IKKβ mRNA, VEGF were decreased （P〈0.01）. Conclusion： Weiyanyihao could inhibit the activation of NF-κB and VEGF pathway, to reduce the expression of NF-κBp65, IKKβ mRNA, VEGF, which could inhibit chronic inflammation and angiogenesis in gastric mucosa.

关 键 词：胃炎Ⅰ号 胃癌前病变 NF-ΚB IKKΒ MRNA VEGF 

分 类 号：R285.5[医药卫生—中药学]