软组织支架3D打印/压印成形工艺研究  

3D Printing/Imprinting Molding Process of Soft Tissue Scaffolds

作　　者：王振[1] 王亚宁[1] 陈冬[1] 郑兆波 刘亚雄[1] 贺健康[1] 王玲[1] 李涤尘[1] 靳忠民[1] 

机构地区：[1]西安交通大学机械制造系统工程国家重点实验室,西安710049

出　　处：《西安交通大学学报》2015年第8期141-146,共6页Journal of Xi'an Jiaotong University

基　　金：国家自然科学基金资助项目(51275387);中央高校基本科研业务费专项资金资助项目

摘　　要：针对当前采用3D打印技术制作软组织工程支架效率低、对细胞损伤大、圆形流道成形精度低等问题,提出了压印与打印技术相结合的直接成形新方法。首先采用压印方法结合光交联固化方法来制造具有流道结构的单层软组织支架,之后利用打印方法沿着流道注入明胶等温敏材料,通过低温环境使流道内的明胶等温敏材料固化起支撑作用,并逐层累积形成立体结构,最后利用温度控制使流道内的明胶融化,形成管道,最终形成具有流道结构的软组织支架。针对该工艺,研制开发出了相应自动化成形设备,结果表明,当扫描间距为51mm时,光强均匀度最高,用该工艺制作出来的支架,圆弧形流道结构清晰平滑,流道相通性很好。研究结果有利于软组织工程支架微结构的精确制造,可望进一步用于细胞打印并提高细胞成活率和成形效率。Focusing on the low efficiency, lager cell damage and low forming accuracy oi circular channel in soft tissue scaffolds construction by 3D printing technology, a novel method is presented to fabricate scaffolds by combining imprinting with 3D printing techniques. The imprinting technique is combined with light erosslinking method to make single-layer soft tissue scaffold with flow channel structure. Then, gelatin, a kind of temperature-sensitive material, is injected along the flow channel by printing technique to cure in the low temperature environment to support the upper layers. A stereoscopic structure is formed via the layer-by-layer accumulation. The gelatin is finally melted in the flow channel by temperature control to form pipelines. An automatic fabrication device is developed to realize the strategy. The results demonstrate that the light uniformity reaches the maximum when the scanning interval reaches 51 mm, and that the arc-type flow channel structure is clearly visible and interlinked. The approach is beneficial to accurate manufacture of soft tissue scaffolds, and it can be expected to improve cell survival rate and forming efficiency in cell printing.

关 键 词：流道结构 压印/打印成形 分层制造 软组织工程 

分 类 号：Q811.2[生物学—生物工程]