湖北省现行家蚕品种原原种AFLP指纹图谱的构建  

The Construction of AFLP Fingerprints among Stocks of Current Silkworm(Bombyx mori)Varieties in Hubei Province

作　　者：吴凡[1] 李德臣[1] 赵春晓[2] 陈登松[3] 

机构地区：[1]湖北省农业科学院经济作物研究所,武汉430070 [2]安康市蚕桑研究所,陕西安康725019 [3]湖北省农业科学院农业质量标准与检测技术研究所,武汉430070

出　　处：《北方蚕业》2015年第2期4-8,共5页North Sericulture

摘　　要：利用AFLP分子标记技术,构建了湖北省10个优质强健原原种的AFLP指纹图谱,分析了其遗传相似系数及亲缘关系。利用4对AFLP引物,共扩增了584条带,其中有多态性带354条,多态性比例为60.62%,构建了其AFLP指纹图谱,可以在分子水平上对这10个家蚕原原种进行鉴定和检测。The analysis on genetic diversity and phylogenetic relationship among 10 Bombyx mori（silkworm）varieties cur-rently used in Hubei province was carried out based on AFLP molecular markers. 4 primer-pair of AFLP were screenedout, and 584 nucleotide fragments were amplified among which 354 polymorphism nucleotides were found, with a poly-morphism rate about 60.62%. The AFLP fingerprints were conducted, which can be used to identify 10 Bombyx mori va-rieties. The results provide a theoretical basis for the protection of silkworm varieties.

关 键 词：家蚕 AFLP 指纹图谱 品种鉴定 

分 类 号：S882[农业科学—特种经济动物饲养]