高效液相色谱法测定醒酒护肝口服液中槲皮素含量  

Quantitative Determination of Quercetin in Xingjiuhugan Oral Solution through HPLC

作　　者：李庆德[1] 郑锦坤[1] 李旭桂[1] 黄红雯 张毅[3] 卞益民[1] 罗启年[1] 

机构地区：[1]广东省粤北人民医院,广东韶关512000 [2]广东省韶关市食品药品检验所,广东韶关512000 [3]丽珠集团利民制药厂药物研究所,广东韶关512000

出　　处：《中国药业》2015年第15期32-33,共2页China Pharmaceuticals

基　　金：广东省2011年建设中医药强省立项资助科研课题;项目编号:20111066

摘　　要：目的建立测定醒酒护肝口服液中槲皮素含量的高效液相色谱（HPLC）法。方法色谱柱为Kromasil C18柱（250 mm×4.6 mm,5μm）,以甲醇-0.4%磷酸溶液（50∶50）为流动相,检测波长360 nm,柱温25℃。结果槲皮素进样量在0.109 8～0.549 0μg范围内与峰面积呈良好的线性关系（r=0.999 5）。平均回收率为98.26%,RSD为0.61%（n=6）。结论该法操作简便、快速、重复性好,可用于醒酒护肝口服液的质量控制。Objective To investigate quantitative determination method of quercetin in Xingjiuhugan oral solution to provide identifiable ground for the foundation of quality standards. Methods Octadecylsilane chemically bonded silica column was adopted with methanol -0. 4% phosphate（50 ： 50） as flowing phase, testing wave 360 nm, column temperature 25 ℃ . Results A good linear relationship of Quercetin showed between 0. 109 8 - 0. 549 0 μg（ r = 0. 999 5）. Lower limit of average recovery was 98. 26% ,RSD = 0. 61% （ n = 6）. Conclusion This method is simple, rapid, reproducible and can be used for the quality control of Xingjiuhugan oral liquid.

关 键 词：醒酒护肝口服液 槲皮素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]