高效液相色谱法测定健阳片中淫羊藿苷含量  

Determination of Icariin Contents in Jianyang Tablets by HPLC

作　　者：谭安军 

机构地区：[1]重庆市永川区中医院,重庆402160

出　　处：《中国药业》2015年第15期34-35,共2页China Pharmaceuticals

摘　　要：目的建立测定健阳片中淫羊藿苷含量的高效液相色谱（HPLC）法。方法色谱柱为Waters Symmetry C18柱（250 mm×4.6 mm,5μm）,以乙腈-水（30∶70）为流动相,检测波长270 nm。结果淫羊藿苷进样量在0.24～2.40μg范围内与峰面积线性关系良好,加样回收率为98.26%,RSD为1.02%（n=6）。结论 HPLC法简便、准确,可用于该制剂的质量控制。Objective To determine the contents of Icariin in Jianyang tablets. Methods HPLC method was adopted a Waters Symmetry C18 column （250 mm ＆#215; 4. 6 mm, 5 μm ） was used. The mobile phase was acetonitrile - water （ 30 ： 70 ） . The detection wavelength was at 270 nm. Results The calibration curve of Icariin was linear in the range of 0. 24 - 2. 40 g（ r = 0. 999 8）, the averge of recovery was 98. 26% , RSD = 1. 02% （ n = 6）. Conclusion The methods is simple and accurate, It can be used for quality control of Jianyang tablets.

关 键 词：淫羊藿苷 健阳片 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]