新藤黄酸对人肺癌细胞株A549的增值和凋亡的影响  

Effects of gambogenic acid on proliferation and apoptosis of A549 lung cancer cells

作　　者：范从进[1] 段惠芳[1] 

机构地区：[1]南京军区庐山疗养院,江西九江332000

出　　处：《国际检验医学杂志》2015年第15期2180-2182,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨新藤黄酸(GNA)对人肺癌细胞株A549的增值和凋亡的影响及其调控的可能机制。方法用不同浓度的GNA处理A549细胞,分为对照组和GNA组(GNA浓度分别为0.5、1.5、2.0mg/L),采用MTT、细胞划痕、Hoechst染色法观察细胞的增殖、迁移和凋亡状况;Western blot检测凋亡相关蛋白caspase3、caspase9、p53和NF-κB的表达变化。结果 GNA能明显抑制细胞的体外增殖和迁移且呈剂量依赖性;Hoechst染色显示GNA能诱导细胞凋亡,且随着浓度的升高凋亡现象越来越明显;Western blot显示GNA能上调促进凋亡的蛋白caspase3、caspase9、p53和下调NF-κB的表达(P<0.05)。结论 GNA能明显抑制人肺癌细胞株A549的增殖和迁移并诱导凋亡。Objective To investigate the effects of gambogenic acid（GNA） on the proliferation and apoptosis of A549 lung canc‐er cells and its possible mechanism of regulation .Methods A549 cells were treated with different concentrations of GNA ,which di‐vided into control group and GNA group（0 .5 ,1 .5 ,20 mg/L） .The cell proliferation and apoptosis of A549 was analyzed by MTT , Hoechst staining ;The expression of apoptosis‐related protein caspase3 ,caspase9 ,p53 and NF‐κB were studied by Western blot .Re‐sults GNA could inhibit growth of A549 cells ;Moreover GNA could also induce apoptosis with the concentration increased more obviously ;Western blot showed that GNA down‐regulate NF‐κB ,increased caspase3 ,caspase9 and p53 expression（P〈0 .05） .Con‐clusion GNA can efficiently promote the cell proliferation inhibition and the apoptosis of A 549 cells .

关 键 词：新藤黄酸 凋亡 表达 

分 类 号：R273[医药卫生—中西医结合]